Khalili Mahdi, Ekhlasi Aryan, Solouk Atefeh, Haghbin Nazarpak Masoumeh, Akbari Somaye
Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic) Tehran Iran
New Technologies Research Center (NTRC), Amirkabir University of Technology (Tehran Polytechnic) Tehran Iran
RSC Adv. 2025 Mar 3;15(9):6902-6913. doi: 10.1039/d4ra08014a. eCollection 2025 Feb 26.
The human amniotic membrane (hAM) is a biological material widely utilized to mimic the extracellular matrix in damaged skin. Despite its potential, clinical applications of hAM have been hindered by its poor mechanical properties. Furthermore, cryopreservation process used to store hAM could compromise its inherent bactericidal properties. This study explores an innovative approach by combining hAM with 2, 4, 6 and 8% w/v of gelatine (Gel) and incorporating 100, 500 and 1000 μL of poly(propylene imine) (PPI) dendrimer-protected silver nanoparticles (AgNPs) to create antibacterial-bolstered scaffolds using freeze-drying technique. Based on results, hAM/Gel2/S500 scaffold was identified as optimal specimen. It exhibited favorable properties, including an ultimate tensile strength of 16 kPa, an elastic modulus of 26.66 kPa, an elongation at break of 59.60%, an average pore size of 490 μm and a porosity of 52.93%. degradation indicated that degradation rate of the scaffold was 30% lower on the 1st day and 20% higher on the 21st day compared to commercial ChitoHeal dressing. It also demonstrated higher water absorbance of 100 and 139% at 1 and 48 hours, respectively, compared to ChitoHeal dressing. Additionally, uniform distribution of AgNPs throughout the scaffold and their release from 2.30 μg mL on the 1st day to 10.40 μg mL by the 3rd day, resulted in an elevated inhibition zone against and . Finally, all antibacterial-bolstered scaffolds exhibited 85-89% cell viability after 24 hours and 80-83% after 72 hours. Consequently, hAM/Gel2/S500 scaffold showed promising results for application in wound healing.
人羊膜（hAM）是一种被广泛用于模拟受损皮肤细胞外基质的生物材料。尽管其具有潜力，但hAM的临床应用因其较差的机械性能而受到阻碍。此外，用于储存hAM的冷冻保存过程可能会损害其固有的杀菌性能。本研究探索了一种创新方法，即将hAM与2%、4%、6%和8% （w/v）的明胶（Gel）相结合，并加入100、500和1000 μL的聚（丙烯亚胺）（PPI）树枝状大分子保护的银纳米颗粒（AgNPs），采用冷冻干燥技术制备抗菌增强支架。基于结果，hAM/Gel2/S500支架被确定为最佳样本。它表现出良好的性能，包括16 kPa的极限拉伸强度、26.66 kPa的弹性模量、59.60%的断裂伸长率、490 μm的平均孔径和52.93%的孔隙率。降解表明，与市售的ChitoHeal敷料相比，该支架在第1天的降解率低30%，在第21天高20%。与ChitoHeal敷料相比，它在1小时和48小时的吸水率也分别更高，为100%和139%。此外，AgNPs在整个支架中均匀分布，其释放量从第1天的2.30 μg/mL到第3天的10.40 μg/mL，导致对[具体细菌1]和[具体细菌2]的抑菌圈增大。最后，所有抗菌增强支架在24小时后细胞活力为85 - 89%，72小时后为80 - 83%。因此，hAM/Gel2/S500支架在伤口愈合应用中显示出有前景的结果。
