Ramachandran Swetha Parakkulathil, Jayanthikumari Vivek Padmanabhan, Kodavanthodi Fasna, Saraladevi Resmi Mohankumar
Post-Graduate and Research Department of Botany, Sree Neelakanta Government Sanskrit College, University of Calicut, Pattambi, Palakkad (Dt), Kerala, India.
Folia Microbiol (Praha). 2025 Mar 4. doi: 10.1007/s12223-025-01254-8.
Plant growth-promoting endophytes (PGPE) are microorganisms which reside in plant tissues and are beneficial to the host in plant growth promotion and pathogen resistance. They are the eco-friendly and sustainable alternative to chemical fertilizers and pesticides. This study aimed to analyze the plant growth-promoting properties of the five endophytic fungal strains from the medicinal plant Aegle marmelos Corr. and evaluate their effects on Oryza sativa plants. Firstly, endophytes were isolated from the different parts of A. marmelos and identified by ITS sequencing. Phosphate solubilization ability was checked in Pikovskaya's agar medium, IAA secretion was measured by the Salkowski colourimetric method, and ACC deaminase activity was checked by Penrose's method. Four endophytic fungal strains with promising PGP activities were inoculated into rice seeds to check their growth promotion in rice. The strain Purpureocillium lilacinum (AMR2) enhanced the seed vigour of rice seeds and demonstrated excellent root colonization ability. Periconia byssoides (AML2) and Medicopsis romeroi (AMS3) were the most effective plant growth-promoting agents, leading to both crop yield improvement and enhanced plant morphological growth due to their great ability to solubilize inorganic phosphate, ACC deaminase activity and production of IAA and Gibberellin A3 (GA3). These endophytic strains could serve as microbial inoculants to enhance crop production, offering an eco-friendly alternative.
植物促生内生菌（PGPE）是存在于植物组织中、对宿主植物生长促进和抗病性有益的微生物。它们是化肥和农药的环保且可持续的替代品。本研究旨在分析从药用植物印度枳椇（Aegle marmelos Corr.）中分离出的5株内生真菌菌株的植物促生特性，并评估它们对水稻植株的影响。首先，从印度枳椇的不同部位分离内生菌，并通过ITS测序进行鉴定。在Pikovskaya琼脂培养基中检测解磷能力，用Salkowski比色法测定吲哚乙酸（IAA）分泌量，用Penrose法检测1-氨基环丙烷-1-羧酸（ACC）脱氨酶活性。将4株具有良好植物促生活性的内生真菌菌株接种到水稻种子中，以检测它们对水稻生长的促进作用。淡紫紫孢菌（Purpureocillium lilacinum，AMR2）菌株提高了水稻种子的活力，并表现出优异的根部定殖能力。葡萄穗霉（Periconia byssoides，AML2）和罗氏拟盘多毛孢（Medicopsis romeroi，AMS3）是最有效的植物促生剂，由于它们具有很强的溶解无机磷、ACC脱氨酶活性以及产生IAA和赤霉素A3（GA3）的能力，从而提高了作物产量并促进了植物形态生长。这些内生菌株可作为微生物接种剂来提高作物产量，提供了一种环保的替代方案。
