Wu Luling, Li Zilu, Wang Kun, Groleau Robin R, Rong Xiaodi, Liu Xueting, Liu Caiyun, Lewis Simon E, Zhu Baocun, James Tony D
Department of Chemistry, University of Bath, Bath BA2 7AY, U.K.
School of Water Conservancy and Environment, University of Jinan, Jinan 250022, China.
J Am Chem Soc. 2025 Mar 19;147(11):9001-9018. doi: 10.1021/jacs.4c17092. Epub 2025 Mar 4.
Liver disease poses a significant challenge to global health, and its early diagnosis is crucial for improving treatment outcomes and patient prognosis. Since fluctuation of key biomarkers during the onset and progression of liver diseases can directly reflect liver health and normal/abnormal function, biomarker-based assays are vital tools for the early detection of liver disease. In this context, small molecule fluorescent probes have undeniably emerged as indispensable tools for diagnosis and analysis, with an ever-growing number of small molecule-based fluorescent probes being developed over recent years, with the sole aim of monitoring relevant biomarkers of liver disease. This perspective will focus on the development and application of probes developed primarily over the last 10 years for diagnosing a range liver disease-related processes. It will outline the foundational design strategies for developing promising probes, their optical response to key biomarkers, and how they have been demonstrated in proof-of-concept imaging applications. Current challenges and new developments in the field will be discussed, with the aim of providing insights and highlighting opportunities in the field.
肝脏疾病对全球健康构成重大挑战，其早期诊断对于改善治疗效果和患者预后至关重要。由于关键生物标志物在肝脏疾病发生和发展过程中的波动可直接反映肝脏健康状况以及正常/异常功能，基于生物标志物的检测方法是早期检测肝脏疾病的重要工具。在此背景下，小分子荧光探针已无可争议地成为诊断和分析不可或缺的工具，近年来基于小分子的荧光探针数量不断增加，其唯一目的是监测肝脏疾病的相关生物标志物。本综述将聚焦于主要在过去10年开发的用于诊断一系列肝脏疾病相关过程的探针的开发与应用。它将概述开发有前景探针的基础设计策略、它们对关键生物标志物的光学响应，以及它们在概念验证成像应用中的展示方式。还将讨论该领域当前的挑战和新进展，旨在提供见解并突出该领域的机遇。
