储存条件对牛载体红细胞的影响。
Effects of storage conditions on bovine carrier erythrocytes.
作者信息
DeLoach J R
出版信息
Am J Vet Res. 1985 May;46(5):1092-4.
PMID:4003885
Abstract
Bovine carrier RBC containing [14C]sucrose as a marker were stored for 23 days in protein-free media. Cell recovery was highest with a phosphate buffer (pH 7.0) containing 2 mM MgCl2, 5 mM inosine, 5 mM adenosine, and 10 mM glucose. A cell recovery of 60% was observed after 23 days of storage at 4 C. Stored carrier erythrocytes reinjected into cattle had an 18-day half-life, which is less than that for fresh carrier RBC.
摘要
含有[14C]蔗糖作为标记物的牛载体红细胞在无蛋白培养基中储存23天。在含有2 mM氯化镁、5 mM肌苷、5 mM腺苷和10 mM葡萄糖的磷酸盐缓冲液（pH 7.0）中，细胞回收率最高。在4℃储存23天后，观察到细胞回收率为60%。重新注入牛体内的储存载体红细胞的半衰期为18天，这比新鲜载体红细胞的半衰期短。
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