Valeri C R, Lindberg J R, Contreras T J, Lowrie G B, Pivacek L E, Gray A, Valeri D A, Emerson C P
Am J Vet Res. 1981 Sep;42(9):1590-4.
Nonrejuvenated and rejuvenated baboon RBC were freeze-preserved with 40% (w/v) glycerol at -80 C. To prepare rejuvenated RBC, a 50-ml solution containing pyruvate, inosine, glucose, phosphate, and adenine was used and RBC were incubated with this solution before glycerolization and freezing. Appropriate steps were taken to minimize osmotic damage to the RBC during glycerolization and deglycerolization. Nonrejuvenated and rejuvenated cryopreserved RBC had freeze-thaw recovery values of 98%, freeze-thaw-wash recovery values of 92%, and 24-hour post-transfusion survival values of 85%. Some units of cryopreserved RBC were autotransfused after thawing, washing, and storage at 4 C for 24 hours. Other units were perfused in vitro before autotransfusion. After 24 hours of postwash storage, the RBC were concentrated by centrifugation and suspended in a plasma protein fraction and/or an electrolyte solution, and then were exposed to extracorporeal perfusion. Serious adverse effects were not observed on posttransfusion survival, function, or hemolysis in nonrejuvenated or rejuvenated baboon RBC as a result of perfusion in vitro.
未复壮和复壮的狒狒红细胞在-80℃下用40%（w/v）甘油冷冻保存。为制备复壮红细胞，使用了一种含有丙酮酸、肌苷、葡萄糖、磷酸盐和腺嘌呤的50毫升溶液，在甘油化和冷冻之前，将红细胞与该溶液孵育。采取了适当步骤以尽量减少甘油化和去甘油化过程中对红细胞的渗透损伤。未复壮和复壮的冷冻保存红细胞的冻融恢复值为98%，冻融洗涤恢复值为92%，输血后24小时存活值为85%。一些冷冻保存的红细胞单位在解冻、洗涤并在4℃储存24小时后进行自体输血。其他单位在自体输血前进行体外灌注。洗涤后储存24小时后，通过离心浓缩红细胞并悬浮于血浆蛋白组分和/或电解质溶液中，然后进行体外灌注。未复壮或复壮的狒狒红细胞经体外灌注后，在输血后存活、功能或溶血方面未观察到严重不良反应。
