Aryati Yani, Farastuti Ekorini, Sholichah Lili, Koesharyani Isti, Gardenia Lila, Septiningsih Early, Yamin Muhamad, Oryzanti Parwa, Puspaningsih Dewi, Sugiani Desy
Research Center for Applied Microbiology, National Research and Innovation Agency, Jl. Raya Jakarta-Bogor Km. 46, Cibinong 16911, West Java, Selatan, 15418, Indonesia.
Faculty of Agriculture, Djuanda University, Jagorawi Toll Rd No.1, Ciawi, Bogor Regency, West Java 16720, Indonesia.
Vet World. 2025 Jan;18(1):228-237. doi: 10.14202/vetworld.2025.228-237. Epub 2025 Jan 30.
Prebiotics, such as saccharides in honey, play a crucial role in improving gut microbiota, digestion, and immune function. This study evaluates the effects of Kapok flower honey saccharides on growth performance, digestive enzyme activity, intestinal morphology, and gut microbiota in common carp ().
A completely randomized design was implemented with four honey supplementation levels (0% control, 0.5%, 0.75%, and 1%) applied to juvenile diets over 30 days. Growth performance, feed utilization, intestinal microvilli structure, gut microbiota, and amylase activity were analyzed using advanced techniques, including high performance liquid chromatography, scanning electron microscopy, and biochemical assays.
Kapok flower honey contains fructooligosaccharides (FOS, 14.76%) and inulin (6.6%). Supplementation at 1% significantly improved weight gain, feed conversion ratio, and specific growth rate. Amylase activity increased with honey supplementation, peaking at 24.13 ± 3.11 U g protein for the 1% group. Gut morphology analysis revealed longer, denser intestinal microvilli and higher perimeter ratios in honey-treated groups than controls. Microbiota analysis showed increased beneficial spp. exclusively in the honey-supplemented groups.
Honey saccharides, particularly FOS and inulin, significantly enhance the growth performance, digestive enzyme activity, and gut health of common carp. Supplementation with 1% honey is optimal, improving feed efficiency and fostering beneficial gut microbiota. These findings highlight honey as a cost-effective, natural prebiotic for aquaculture.
益生元，如蜂蜜中的糖类，在改善肠道微生物群、消化和免疫功能方面发挥着关键作用。本研究评估了木棉花蜂蜜糖类对鲤鱼生长性能、消化酶活性、肠道形态和肠道微生物群的影响。
采用完全随机设计，在30天内将四种蜂蜜添加水平（0%对照、0.5%、0.75%和1%）应用于幼鱼饲料中。使用先进技术，包括高效液相色谱、扫描电子显微镜和生化分析，分析生长性能、饲料利用率、肠道微绒毛结构、肠道微生物群和淀粉酶活性。
木棉花蜂蜜含有低聚果糖（FOS，14.76%）和菊粉（6.6%）。1%的添加量显著提高了体重增加、饲料转化率和特定生长率。淀粉酶活性随蜂蜜添加量增加而增加，1%组达到峰值24.13±3.11 U g蛋白质。肠道形态分析显示，蜂蜜处理组的肠道微绒毛比对照组更长、更密集，周长比更高。微生物群分析表明，仅在添加蜂蜜的组中有益菌增加。
蜂蜜糖类，特别是FOS和菊粉，可以显著提高鲤鱼的生长性能、消化酶活性和肠道健康。添加1%的蜂蜜效果最佳，可提高饲料效率并促进有益肠道微生物群的生长。这些发现突出了蜂蜜作为水产养殖中一种经济高效的天然益生元原料的作用。
