Wallis C, Melnick J L
Appl Environ Microbiol. 1985 May;49(5):1251-3. doi: 10.1128/aem.49.5.1251-1253.1985.
A new semiautomated instrument is described which quantifies the number of bacteria in potable waters within 3 min, providing a permanent colorimetric record of the results. The bacteria detection device can measure as few as 100 CFU/ml in potable waters. In brief, a 100- to 1,000-ml sample of tap water is drawn through a large surface, customized filter housed in the device, and bacteria, rust, and humic acid in the water are concentrated thereon. A reducing agent is used to remove the rust and humic acid from the filter. The filter is inverted and backflushed to elute the bacteria which are collected and reconcentrated onto a 7-mm-diameter filter surface. The reconcentrated bacteria are stained, and the filter fibers are preferentially decolorized without removing the dye from the bacteria. The color intensity of the filter surface is compared with a color guide to determine the amount of bacteria in the test water.
本文介绍了一种新型半自动仪器，该仪器可在3分钟内对饮用水中的细菌数量进行定量，并提供结果的永久性比色记录。该细菌检测装置可检测低至100 CFU/ml的饮用水。简而言之，抽取100至1000毫升的自来水样本，使其通过该装置中一个大表面积的定制过滤器，水中的细菌、铁锈和腐殖酸会浓缩在过滤器上。使用一种还原剂去除过滤器上的铁锈和腐殖酸。将过滤器倒置并进行反冲洗，以洗脱收集到的细菌，并将其重新浓缩到一个直径7毫米的过滤器表面上。对重新浓缩后的细菌进行染色，优先使过滤器纤维脱色，同时不会从细菌上洗脱染料。将过滤器表面的颜色强度与色卡进行比较，以确定测试水中细菌的数量。
