Thomason B M, Wells J G
Appl Microbiol. 1971 Nov;22(5):876-84. doi: 10.1128/am.22.5.876-884.1971.
A polyvalent OH conjugate for Salmonella O groups A through I, K, L, and O was prepared and tested against pure cultures of salmonellae, nonsalmonellae, and a variety of food, fecal, and environmental specimens. Examination of pure cultures revealed that the conjugate gave negligible staining with representative strains of Shigella, Proteus, Providence, Serratia, and Pseudomonas. However, it stained 12% of the Escherichia coli and Citrobacter freundii strains and 36% of the Arizona strains. Over 1,200 specimens of various types were examined by both fluorescent-antibody (FA) and cultural procedures. Results indicate that, when used with discretion, FA screening can be a useful tool for rapid presumptive indication of the presence of salmonellae. The need for careful selection of strains used for preparing antisera and the importance of adequate evaluation of Salmonella FA reagents are discussed.
制备了一种针对沙门氏菌O群A至I、K、L和O的多价OH缀合物，并针对沙门氏菌、非沙门氏菌的纯培养物以及各种食品、粪便和环境标本进行了测试。对纯培养物的检查表明，该缀合物与志贺氏菌、变形杆菌、普罗威登斯菌、沙雷氏菌和假单胞菌的代表性菌株染色可忽略不计。然而，它对12%的大肠杆菌和弗氏柠檬酸杆菌菌株以及36%的亚利桑那菌株进行了染色。通过荧光抗体（FA）和培养程序对1200多个不同类型的标本进行了检查。结果表明，谨慎使用时，FA筛查可作为快速推定沙门氏菌存在的有用工具。讨论了仔细选择用于制备抗血清的菌株的必要性以及充分评估沙门氏菌FA试剂的重要性。
