Xia Chunmei, Xu Xiang, Zhang Ruifen, Su Dongxiao, Jia Xuchao, Deng Mei, Lee Yuan-Kun, Zhang Mingwei, Huang Fei
Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China; College of Food Science & Technology, Huazhong Agricultural University, Wuhan 430070, China.
Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China.
Int J Biol Macromol. 2025 May;306(Pt 3):141711. doi: 10.1016/j.ijbiomac.2025.141711. Epub 2025 Mar 3.
The gastrointestinal digestion and fermentation in vitro of longan polysaccharide (LP) and its degraded products (DLP-1, DLP-4, and DLP-8) with different molecular weights (Mw) were investigated. Results indicated that four polysaccharides were only slightly degraded in the gastrointestinal digestion and mainly utilized by gut microbiota in the colon. Among them, DLP-8 with the lowest Mw was easily utilized by intestinal bacteria accompanied by the highest production of acetic and butyric acids. The DLP-8 group exhibited the best growth of beneficial bacteria including Dialister, Bifidobacterium, and Bacteroides. Correlation analysis further verified that the modulatory effect of longan polysaccharide on gut microbiota was structure-dependent, and Mw performed a key role in selectively regulating gut microbial composition. These results showed the smaller Mw longan polysaccharides were more readily fermented leading to modulation of gut microbiota in vitro.
研究了不同分子量（Mw）的龙眼多糖（LP）及其降解产物（DLP-1、DLP-4和DLP-8）在体外的胃肠消化和发酵情况。结果表明，四种多糖在胃肠消化中仅轻微降解，主要被结肠中的肠道微生物群利用。其中，Mw最低的DLP-8容易被肠道细菌利用，同时乙酸和丁酸的产量最高。DLP-8组中包括戴阿李斯特菌、双歧杆菌和拟杆菌在内的有益菌生长最佳。相关性分析进一步证实，龙眼多糖对肠道微生物群的调节作用取决于结构，分子量在选择性调节肠道微生物组成中起关键作用。这些结果表明，分子量较小的龙眼多糖更容易发酵，从而在体外调节肠道微生物群。
