Xu Qingrui, Lv Yiyi, Yuan Xiaohui, Huang Guichun, Guo Zhongxia, Tan Jiana, Qiu Shuyi, Wang Xiaodan, Wei Chaoyang
Key Laboratory of Fermentation Engineering and Biological Pharmacy of Guizhou Province, School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China.
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro-Bioengineering, College of Life Sciences, Guizhou University, Guiyang 550025, China.
Foods. 2025 Apr 27;14(9):1529. doi: 10.3390/foods14091529.
, an underutilized wild plant, has been found to have a high nutritional value. This study used simulated digestion and fecal fermentation models to investigate the digestive properties of the purified acidic pectin polysaccharide of and its impact on the gut microbiota and metabolites. polysaccharide (PFP) is mainly composed of rhamnose (Rha), galacturonic acid (GalA), glucose (Glc), galactose (Gal), and arabinose (Ara), with a molecular weight (Mw) of 851.25 kDa. Following simulated digestion, the Mw of PFP remained consistent. The reduced sugar content showed minimal change, suggesting that PFP exhibits resistance to gastrointestinal digestion and can effectively reach the colon. Following fecal fermentation, the molecular weight, monosaccharide, and carbohydrate contents of PFP decreased, while the short-chain fatty acid content increased. This suggests that PFP is susceptible to degradation by microorganisms and can be metabolized into acetic acid and -butyric acid, contributing to the regulation of intestinal health. Meanwhile, PFP promotes the reproduction of beneficial bacteria such as , , and , inhibits the growth of harmful bacteria like , and generates metabolites such as thiamine, leonuriside A, oxoadipic acid, S-hydroxymethylglutathione, and isonicotinic acid, which exert beneficial effects on human health. These results indicate that PFP has great potential in regulating the gut microbiota and generating beneficial metabolites to promote intestinal functional health and can be used as a prebiotic to prevent diseases by improving intestinal health.
一种未得到充分利用的野生植物，已被发现具有很高的营养价值。本研究使用模拟消化和粪便发酵模型，研究了该植物纯化酸性果胶多糖的消化特性及其对肠道微生物群和代谢物的影响。该植物多糖（PFP）主要由鼠李糖（Rha）、半乳糖醛酸（GalA）、葡萄糖（Glc）、半乳糖（Gal）和阿拉伯糖（Ara）组成，分子量（Mw）为851.25 kDa。模拟消化后，PFP的Mw保持一致。还原糖含量变化极小，表明PFP对胃肠道消化具有抗性，能够有效到达结肠。粪便发酵后，PFP的分子量、单糖和碳水化合物含量降低，而短链脂肪酸含量增加。这表明PFP易被微生物降解，并可代谢为乙酸和丁酸，有助于调节肠道健康。同时，PFP促进了如双歧杆菌、嗜酸乳杆菌和粪肠球菌等有益菌的繁殖，抑制了如大肠杆菌等有害菌的生长，并产生了硫胺素、益母草苷A、氧代己二酸、S-羟甲基谷胱甘肽和异烟酸等代谢物，这些对人体健康发挥有益作用。这些结果表明，PFP在调节肠道微生物群和产生有益代谢物以促进肠道功能健康方面具有巨大潜力，并且可以用作益生元，通过改善肠道健康来预防疾病。
