Li Yuxiang, Ma Yixiang, Liu Xinrong, Guo Ya, Ji Ning, Li Zhaofei
State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Taicheng Road, 712100, Yangling, Shaanxi, China.
Arch Virol. 2025 Mar 5;170(4):65. doi: 10.1007/s00705-025-06266-w.
Using degenerate primers and an overlap PCR approach, we identified a new densovirus (family Parvoviridae, subfamily Densovirinae) in an asexual clone of the leaf-curl plum aphid, Brachycaudus helichrysi, collected in Baoji, China, in June 2021. A nearly complete genome sequence of 5,551 nucleotides (nt) was obtained. The virus, with the proposed name "Brachycaudus helichrysi densovirus" (BhDV), has an ambisense genome organization with two ORFs in the sense strand encoding the nonstructural proteins NS1 and NS2 and one ORF at the antisense strand encoding the virion capsid protein VP. These proteins share 37-70% and 10-33% amino acid sequence identity with homologs of the other aphid densoviruses and non-aphid densoviruses, respectively. Phylogenetic analysis suggested that this virus is a member of a new species in the genus Hemiambidensovirus.
利用简并引物和重叠PCR方法，我们在2021年6月于中国宝鸡采集的卷叶李短尾蚜无性克隆体中鉴定出一种新的浓核病毒（细小病毒科，浓核病毒亚科）。获得了一个长度为5551个核苷酸（nt）的近乎完整的基因组序列。该病毒，暂定名为“卷叶李短尾蚜浓核病毒”（BhDV），具有双义基因组结构，正义链上有两个开放阅读框（ORF），分别编码非结构蛋白NS1和NS2，反义链上有一个ORF，编码病毒粒子衣壳蛋白VP。这些蛋白与其他蚜虫浓核病毒和非蚜虫浓核病毒的同源物分别具有37 - 70%和10 - 33%的氨基酸序列同一性。系统发育分析表明，该病毒是半双义浓核病毒属一个新物种的成员。
