Yan Kuan, Li Xinyi, Cai Yu, Meng Lina, Wei Qin, Zhao Xianming, M Y Heakel Rania, M Atif Amr, A Abd Elhamid Mohamed, A Soaud Salma, H El-Sappah Ahmed
Faculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, China.
Sichuan Oil Cinnamon Engineering Technology Research Center, Yibin University, Yibin, China.
Front Microbiol. 2025 Feb 19;15:1501057. doi: 10.3389/fmicb.2024.1501057. eCollection 2024.
Bamboo is a sustainable and degradable resource for sustenance, high-strength cellulose microfibers, and synthetic fiber in China. Endophytic bacteria enhance root development and ethylene levels, benefiting the host plants' physiology.
We investigated the population, diversity, and abundance of endophytic bacteria in the leaves of three bamboo species-, , and -using high-throughput 16S rDNA sequencing.
A total of 1,159 operational taxonomic units (OTUs) were obtained and further classified into 26 phyla, 64 classes, 158 orders, 270 families, 521 genera, and 811 species. The phyla with the highest abundance were Proteobacteria, Actinobacteria, and Myxococcota, and the highest genera included 1,174-901-12, , and unclassified_f__Enterobacteriaceae. The relative richness of endophytic bacteria in the three species was in the following order: > > . The PICRUSt functional richness analysis of endophytic bacteria indicated their involvement in six biological pathways: "cellular processes," "environmental information processing," "genetic information processing," "human diseases," "metabolism," and "organic systems." Among the 41 sub-functions, the most common were "amino acid metabolism," "carbohydrate metabolism," "cell motility," "cellular signaling," "energy metabolism," and "membrane transport." Our results provide precise knowledge for better managing bamboo forests and pave the way for isolating secondary metabolites and potential bioactive compounds.
在中国，竹子是一种可持续且可降解的资源，可用于维持生计、制造高强度纤维素微纤维和合成纤维。内生细菌可促进根系发育并提高乙烯水平，对宿主植物的生理机能有益。
我们使用高通量16S rDNA测序技术，研究了三种竹子（、和）叶片中内生细菌的种群、多样性和丰度。
共获得1159个可操作分类单元（OTU），并进一步分为26个门、64个纲、158个目、270个科、521个属和811个种。丰度最高的门是变形菌门、放线菌门和粘球菌门，丰度最高的属包括1174-901-12、和未分类的肠杆菌科。三种竹子中内生细菌的相对丰富度顺序为：> > 。内生细菌的PICRUSt功能丰富度分析表明它们参与了六种生物途径：“细胞过程”、“环境信息处理”、“遗传信息处理”、“人类疾病”、“代谢”和“有机系统”。在41个子功能中，最常见的是“氨基酸代谢”、“碳水化合物代谢”、“细胞运动”、“细胞信号传导”、“能量代谢”和“膜运输”。我们的研究结果为更好地管理竹林提供了精确的知识，并为分离次生代谢产物和潜在生物活性化合物铺平了道路。
