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基于16S rDNA测序的毛竹内生细菌特征及多样性研究

Characteristics and diversity of endophytic bacteria in moso bamboo (Phyllostachys edulis) based on 16S rDNA sequencing.

作者信息

Liu Fang, Yuan Zongsheng, Zhang Xintao, Zhang Guofang, Xie Baogui

机构信息

The School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.

Nanjing Wanchen Biological Technology co., LTD, Nanjing, 211299, Jiangsu, China.

出版信息

Arch Microbiol. 2017 Nov;199(9):1259-1266. doi: 10.1007/s00203-017-1397-7. Epub 2017 Jun 10.

Abstract

To understand the diversity and distribution of endophytic bacteria in moso bamboo (Phyllostachys edulis), we used 16S rDNA sequencing to investigate the characteristics and diversity of endophytic bacteria in different moso bamboo tissues. After 454 pyrosequencing, we obtained 141,269 sequences from seven moso bamboo tissue samples. The taxonomic origins of unique sequences were identified using RDP classifier. The results showed that these sequences belonged to 26 bacterial orders, including the Actinomycetales, Rickettsiales, Burkholderiales, Enterobacteriales, and Rhizobiales. Among these, Enterobacteriales was widely found in all bamboo tissues. Endophytic bacterial communities differed between the moso bamboo shoot and pole. With continuous growth and development, the number of endophytic species in the moso bamboo pole increased gradually.

摘要

为了解毛竹(Phyllostachys edulis)内生细菌的多样性和分布情况,我们采用16S rDNA测序技术研究了不同毛竹组织中内生细菌的特征和多样性。经过454焦磷酸测序,我们从七个毛竹组织样本中获得了141,269条序列。使用RDP分类器确定了独特序列的分类起源。结果表明,这些序列属于26个细菌目,包括放线菌目、立克次氏体目、伯克霍尔德氏菌目、肠杆菌目和根瘤菌目。其中,肠杆菌目在所有竹组织中均广泛存在。毛竹笋和竹杆中的内生细菌群落有所不同。随着毛竹的不断生长和发育,竹杆中内生菌的种类数量逐渐增加。

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