Arkani Mahsa, Babaeipour Valiollah, Mohammadi Reza, Deldar Aliasghar
Research Center of Science and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran.
Prep Biochem Biotechnol. 2025;55(8):955-972. doi: 10.1080/10826068.2025.2472961. Epub 2025 Mar 6.
hemoglobin (VHb), a 15,775 Da oxygen-binding protein, enhances intracellular oxygen transfer, improves the efficiency of oxygen use, and promotes organism growth and the desired metabolite production. Researchers have investigated VHb gene expression for two decades to enhance aerobic biological processes in diverse organisms. This review examines the protein structure, biochemical characteristics, genetic structure, cellular mechanisms of activity, and protein engineering of VHb. It then assesses the impact of VHb expression on the performance improvement of bacteria, fungi, plants, and mammalian cells in various biological processes. Finally, the article specifically discusses the VHb gene expression strategies in .
血红蛋白（VHb）是一种分子量为15,775道尔顿的氧结合蛋白，可增强细胞内的氧传递，提高氧利用效率，并促进生物体生长和所需代谢产物的产生。二十年来，研究人员一直在研究VHb基因表达，以增强不同生物体中的需氧生物过程。本综述考察了VHb的蛋白质结构、生化特性、基因结构、细胞活性机制和蛋白质工程。然后评估了VHb表达对细菌、真菌、植物和哺乳动物细胞在各种生物过程中性能改善的影响。最后，本文具体讨论了……中的VHb基因表达策略。
