Cho Mincheol, Kim Eun Seo, Ryu Tae-Kyung, Choi Inseong, Choi Sung-Wook
Biomedical and Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si 14662, Gyeonggi-do, Republic of Korea.
Department of Neurology, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Micromachines (Basel). 2025 Jan 31;16(2):179. doi: 10.3390/mi16020179.
Microfluidic devices are greatly affected by the materials used. The materials used in previous studies had problems in various aspects, such as processing, adsorption, and price. This study will investigate the materials needed to overcome such problems. Various microfluidic devices based on the perfluorinated compound perfluoropolyether (PFPE) were fabricated and mixed with hydrophilic and amphiphilic monomers, including poly(ethylene glycol) diacrylate, polyethylene glycol monomethacrylate, poly(ethylene glycol) methyl ether methacrylate, acrylic acid, and 2-hydroxyethyl methacrylate. A PFPE-based sheet with a repeating structure of hydrophobic and hydrophilic groups was fabricated. Thus, the hydrophilicity of highly hydrophobic PFPE was enhanced. The fluidic channel was engraved on a PFPE-based sheet using laser cutting and a fabricated microfluidic device. The channels of microfluidic devices are micro-scale (100 µm~300 µm). The lipid nanoparticles and droplets generated through the microfluidic device demonstrated uniform particles continuously.
微流控设备受所用材料的影响很大。先前研究中使用的材料在加工、吸附和价格等各个方面都存在问题。本研究将探究克服此类问题所需的材料。制备了各种基于全氟化合物全氟聚醚（PFPE）的微流控设备，并将其与亲水性和两亲性单体混合，包括聚乙二醇二丙烯酸酯、聚乙二醇单甲基丙烯酸酯、聚乙二醇甲基醚甲基丙烯酸酯、丙烯酸和甲基丙烯酸2-羟乙酯。制备了具有疏水和亲水基团重复结构的基于PFPE的薄片。因此，高度疏水的PFPE的亲水性得到了增强。使用激光切割和制备的微流控设备在基于PFPE的薄片上刻蚀出流体通道。微流控设备的通道为微米级（100 µm~300 µm）。通过微流控设备产生的脂质纳米颗粒和液滴持续显示出均匀的颗粒。
