从人肝脏中鉴定出一种基本的杂合谷胱甘肽S-转移酶。谷胱甘肽S-转移酶δ由两个不同的亚基（B1和B2）组成。
Identification of a basic hybrid glutathione S-transferase from human liver. Glutathione S-transferase delta is composed of two distinct subunits (B1 and B2).
作者信息
Stockman P K, Beckett G J, Hayes J D
出版信息
Biochem J. 1985 Apr 15;227(2):457-65. doi: 10.1042/bj2270457.
Abstract
The purification of a hybrid glutathione S-transferase (B1 B2) from human liver is described. This enzyme has an isoelectric point of 8.75 and the B1 and B2 subunits are distinguishable immunologically and are ionically distinct. Hybridization experiments demonstrated that B1 B1 and B2 B2 could be resolved by CM-cellulose chromatography and have pI values of 8.9 and 8.4 respectively. Transferase B1 B2, and the two homodimers from which it is formed, are electrophoretically and immunochemically distinct from the neutral enzyme (transferase mu) and two acidic enzymes (transferases rho and lambda). Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis demonstrated that B1 and B2 both have an Mr of 26 000, whereas, in contrast, transferase mu comprises subunits of Mr 27 000 and transferases rho and lambda both comprise subunits of Mr 24 500. Antisera raised against B1 or B2 monomers did not cross-react with the neutral or acidic glutathione S-transferases. The identity of transferase B1 B2 with glutathione S-transferase delta prepared by the method of Kamisaka, Habig, Ketley, Arias & Jakoby [(1975) Eur. J. Biochem. 60, 153-161] has been demonstrated, as well as its relationship to other previously described transferases.
摘要
本文描述了从人肝脏中纯化一种杂合谷胱甘肽S-转移酶（B1 B2）的过程。这种酶的等电点为8.75，B1和B2亚基在免疫学上可区分，且离子特性不同。杂交实验表明，B1 B1和B2 B2可通过CM-纤维素色谱法分离，其pI值分别为8.9和8.4。转移酶B1 B2及其形成的两种同型二聚体在电泳和免疫化学上与中性酶（转移酶μ）和两种酸性酶（转移酶ρ和λ）不同。十二烷基硫酸钠/聚丙烯酰胺凝胶电泳表明，B1和B2的Mr均为26000，而相比之下，转移酶μ由Mr为27000的亚基组成，转移酶ρ和λ均由Mr为24500的亚基组成。针对B1或B2单体产生的抗血清与中性或酸性谷胱甘肽S-转移酶无交叉反应。已证明转移酶B1 B2与通过Kamisaka、Habig、Ketley、Arias和Jakoby [（1975年）《欧洲生物化学杂志》60，153 - 161]方法制备的谷胱甘肽S-转移酶δ相同，以及它与其他先前描述的转移酶的关系。
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Am J Hum Genet. 1981 Jan;33(1):36-43.
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Cholic acid binding by anionic glutathione-S-transferase from human liver cytosol.人肝细胞溶质阴离子型谷胱甘肽-S-转移酶与胆酸的结合
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Purification of a new glutathione S-transferase (transferase mu) from human liver having high activity with benzo(alpha)pyrene-4,5-oxide.从人肝脏中纯化一种新的谷胱甘肽S-转移酶（转移酶μ），其对苯并（α）芘-4,5-环氧化物具有高活性。
Biochem Biophys Res Commun. 1981 Jan 30;98(2):512-9. doi: 10.1016/0006-291x(81)90870-6.
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