Jeng Long-Bin, Shih Fu-Ying, Liao Yu-Wen, Shyu Woei-Cherng, Teng Chiao-Fang
Organ Transplantation Center, China Medical University Hospital, Taichung, Taiwan, ROC.
Department of Surgery, China Medical University Hospital, Taichung, Taiwan, ROC.
Br J Cancer. 2025 May;132(9):837-848. doi: 10.1038/s41416-025-02975-w. Epub 2025 Mar 7.
Dendritic cell (DC) vaccine is a promising immunotherapy for hepatocellular carcinoma (HCC) via triggering antigen-specific anti-tumor immunity. Hypoxia contributes to higher level and broader spectrum of antigen expression in tumor cells.
This study aims to compare immunological activity and therapeutic efficacy between hypoxic and normoxic HCC cell line lysate-pulsed DC vaccines.
The results showed that hypoxic HCC cell line lysate-pulsed DC vaccines exhibited a stronger activity in producing interleukin-12 and promoting T cell proliferation and cytotoxicity in vitro. In HCC mice, hypoxic HCC cell line lysate-pulsed DC vaccines displayed a better efficacy in improving survival time and tumor volume and inducing intratumoral cytotoxic T cell infiltration and activation as well as tumor cell apoptosis. Adenylate kinase 4-derived antigens were important for hypoxic HCC cell line lysate-pulsed DC vaccine-elicited T cell killing.
In conclusion, this study demonstrated hypoxic HCC cell line lysate-pulsed DC vaccine as a potential therapeutic strategy for HCC.
树突状细胞（DC）疫苗通过触发抗原特异性抗肿瘤免疫，是一种有前景的肝细胞癌（HCC）免疫疗法。缺氧会导致肿瘤细胞中抗原表达水平更高、范围更广。
本研究旨在比较缺氧和常氧肝癌细胞系裂解物脉冲DC疫苗的免疫活性和治疗效果。
结果显示，缺氧肝癌细胞系裂解物脉冲DC疫苗在体外产生白细胞介素-12以及促进T细胞增殖和细胞毒性方面表现出更强的活性。在肝癌小鼠中，缺氧肝癌细胞系裂解物脉冲DC疫苗在延长生存时间、减小肿瘤体积以及诱导肿瘤内细胞毒性T细胞浸润和激活以及肿瘤细胞凋亡方面显示出更好的疗效。腺苷酸激酶4衍生抗原对缺氧肝癌细胞系裂解物脉冲DC疫苗引发的T细胞杀伤作用很重要。
总之，本研究证明缺氧肝癌细胞系裂解物脉冲DC疫苗是一种潜在的肝癌治疗策略。
