致瘤性和非致瘤性人类体细胞杂种的游离细胞质钙浓度。
The free cytoplasmic calcium concentration of tumorigenic and non-tumorigenic human somatic cell hybrids.
作者信息
Banyard M R, Tellam R L
出版信息
Br J Cancer. 1985 Jun;51(6):761-6. doi: 10.1038/bjc.1985.119.
Abstract
The fluorescent indicator of Ca2+ concentration, quin-2, has been used to measure the concentration of free Ca2+ in the cytoplasm of tumorigenic and non-tumorigenic human somatic cell hybrids. The cell hybrids were derived from the fusion of a HeLa derivative (D98 AH2) and normal human fibroblasts. The calcium concentration of the tumorigenic cell lines was 180 +/- 7nM and the level in the non-tumorigenic cells was 136 +/- 6nM. This difference was statistically highly significant (P less than 0.001). Control experiments are reported which show that the level of 3a2+ measured is not influenced by cell density or by the concentration of quin-2-tetra-(acetoxymethyl)ester used in these experiments. The possible implications of this elevated level of cytoplasmic calcium in tumorigenic cells are discussed.
摘要
钙离子浓度荧光指示剂喹啉-2已被用于测量致瘤性和非致瘤性人类体细胞杂种细胞质中游离钙离子的浓度。这些细胞杂种源自HeLa衍生物（D98 AH2）与正常人成纤维细胞的融合。致瘤性细胞系的钙浓度为180±7nM，非致瘤性细胞中的水平为136±6nM。这种差异在统计学上具有高度显著性（P小于0.001）。报告的对照实验表明，所测量的钙离子水平不受细胞密度或这些实验中使用的喹啉-2-四（乙酰氧基甲基）酯浓度的影响。本文讨论了致瘤性细胞中细胞质钙水平升高的可能影响。
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本文引用的文献
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