Bridges K, Levenson R, Housman D, Cantley L
J Cell Biol. 1981 Aug;90(2):542-4. doi: 10.1083/jcb.90.2.542.
An alteration in the rate of calcium transport appears to be the rate-limiting event for the commitment of murine erythroleukemia (MEL) cells to initiate a program of terminal erythroid differentiation. The dimethyl sulfoxide (DMSO)-induced commitment of MEL cells to erythroid differentiation can be inhibited by treatment of cells with the calcium-chelating agent EGTA. Upon removal of EGTA, cells initiate commitment without the 12-h lag normally observed after treatment with DMSO alone. Treatment of cells with DMSO in the presence of calcium ionophore A23187 causes cells to initiate commitment from time zero with no lag. These results suggest that the lag is the time required for DMSO to alter the calcium transport properties of the cell.
钙转运速率的改变似乎是小鼠红白血病（MEL）细胞启动终末红细胞分化程序的限速事件。用钙螯合剂乙二醇双四乙酸（EGTA）处理细胞可抑制二甲基亚砜（DMSO）诱导的MEL细胞向红细胞分化。去除EGTA后，细胞可启动分化，且没有单独用DMSO处理后通常观察到的12小时延迟。在钙离子载体A23187存在的情况下用DMSO处理细胞，可使细胞从零时开始启动分化，没有延迟。这些结果表明，延迟是DMSO改变细胞钙转运特性所需的时间。
