De Martinis Elaine Cristina Pereira, Alves Virgínia Farias, Pereira Marita Gimenez, Andrade Leonardo Neves, Abichabki Nathália, Abramova Anna, Dannborg Mirjam, Bengtsson-Palme Johan
Ribeirão Preto School of Pharmaceutical Sciences, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Faculdade de Farmácia, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
Front Immunol. 2025 Feb 20;16:1488699. doi: 10.3389/fimmu.2025.1488699. eCollection 2025.
Recent advances in cell culturing and DNA sequencing have dramatically altered the field of human microbiome research. Three-dimensional (3D) cell culture is an important tool in cell biology, in cancer research, and for studying host-microbe interactions, as it mimics the characteristics of the host environment in an system, providing reliable and reproducible models. This work provides an overview of the main 3D culture techniques applied to study interactions between host cells and pathogenic microorganisms, how these systems can be integrated with high-throughput molecular methods, and how multi-species model systems may pave the way forward to pinpoint interactions among host, beneficial microbes and pathogens.
细胞培养和DNA测序的最新进展极大地改变了人类微生物组研究领域。三维（3D）细胞培养是细胞生物学、癌症研究以及研究宿主-微生物相互作用的重要工具，因为它在体外系统中模拟宿主环境的特征，提供可靠且可重复的模型。本文综述了应用于研究宿主细胞与致病微生物之间相互作用的主要3D培养技术，这些系统如何与高通量分子方法相结合，以及多物种模型系统如何为确定宿主、有益微生物和病原体之间的相互作用铺平道路。
