Bossi Alessandra Maria, Casella Sofia, Stranieri Chiara, Marinangeli Alice, Bucciarelli Alessio, Fratta Pasini Anna Maria, Maniglio Devid
Department of Biotechnology, University of Verona, LaStMolCAL Lab, Strada Le Grazie 15, 37134 Verona, Italy.
Department of Biotechnology, University of Verona, LaStMolCAL Lab, Strada Le Grazie 15, 37134 Verona, Italy.
Trends Biotechnol. 2025 May;43(5):1215-1233. doi: 10.1016/j.tibtech.2025.02.002. Epub 2025 Mar 6.
Protein-derived biomaterials are currently underrated as building blocks in molecular imprinting, even though they offer several benefits, such as biocompatibility and safe biodegradability. Gelatin is a biopolymer that can be easily modified with pendant double bonds for polymerization, making it suitable for tissue engineering and biofabrication. In this study, we used gelatin methacryloyl (GelMA) as a building block combined with molecular imprinting technology to create an original class of bioinspired nanotraps specifically capable of sequestering the proinflammatory cytokine interleukin-6 (IL-6). The stability in solution, biocompatibility, and biodegradability of the nanotraps were assessed. The nanotraps were selective and specific for IL-6, showing nanomolar affinity and, when tested in vitro on an inflammation cell model, sequestered IL-6 with a dose-response relationship. Overall, our study shows that protein chemistry-driven molecular imprinting could become more widely used to devise biocompatible functional nanomaterials.
蛋白质衍生的生物材料作为分子印迹的构建单元目前被低估了，尽管它们具有多种优势，如生物相容性和安全的生物降解性。明胶是一种生物聚合物，可通过侧链双键轻松修饰以进行聚合反应，使其适用于组织工程和生物制造。在本研究中，我们使用甲基丙烯酰化明胶（GelMA）作为构建单元，结合分子印迹技术，创建了一类独特的受生物启发的纳米陷阱，专门能够捕获促炎细胞因子白细胞介素-6（IL-6）。评估了纳米陷阱在溶液中的稳定性、生物相容性和生物降解性。纳米陷阱对IL-6具有选择性和特异性，表现出纳摩尔亲和力，并且在炎症细胞模型上进行体外测试时，以剂量反应关系捕获IL-6。总体而言，我们的研究表明，蛋白质化学驱动的分子印迹可更广泛地用于设计生物相容性功能纳米材料。
