Yue Kan, Trujillo-de Santiago Grissel, Alvarez Mario Moisés, Tamayol Ali, Annabi Nasim, Khademhosseini Ali
Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston 02139, MA, USA; Harvard-Massachusetts Institute of Technology Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge 02139, MA, USA.
Biomaterials Innovation Research Center, Division of Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston 02139, MA, USA; Harvard-Massachusetts Institute of Technology Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge 02139, MA, USA; Centro de Biotecnología-FEMSA, Tecnológico de Monterrey at Monterrey, Ave. Eugenio Garza Sada 2501 Sur Col. Tecnológico, CP 64849 Monterrey, Nuevo León, Mexico.
Biomaterials. 2015 Dec;73:254-71. doi: 10.1016/j.biomaterials.2015.08.045. Epub 2015 Aug 28.
Gelatin methacryloyl (GelMA) hydrogels have been widely used for various biomedical applications due to their suitable biological properties and tunable physical characteristics. GelMA hydrogels closely resemble some essential properties of native extracellular matrix (ECM) due to the presence of cell-attaching and matrix metalloproteinase responsive peptide motifs, which allow cells to proliferate and spread in GelMA-based scaffolds. GelMA is also versatile from a processing perspective. It crosslinks when exposed to light irradiation to form hydrogels with tunable mechanical properties. It can also be microfabricated using different methodologies including micromolding, photomasking, bioprinting, self-assembly, and microfluidic techniques to generate constructs with controlled architectures. Hybrid hydrogel systems can also be formed by mixing GelMA with nanoparticles such as carbon nanotubes and graphene oxide, and other polymers to form networks with desired combined properties and characteristics for specific biological applications. Recent research has demonstrated the proficiency of GelMA-based hydrogels in a wide range of tissue engineering applications including engineering of bone, cartilage, cardiac, and vascular tissues, among others. Other applications of GelMA hydrogels, besides tissue engineering, include fundamental cell research, cell signaling, drug and gene delivery, and bio-sensing.
甲基丙烯酰化明胶（GelMA）水凝胶因其合适的生物学特性和可调节的物理特性，已被广泛应用于各种生物医学领域。由于存在细胞附着和基质金属蛋白酶响应肽基序，GelMA水凝胶与天然细胞外基质（ECM）的一些基本特性非常相似，这使得细胞能够在基于GelMA的支架中增殖和扩散。从加工角度来看，GelMA也具有多种用途。它在光照下交联形成具有可调节机械性能的水凝胶。它还可以使用包括微成型、光刻、生物打印、自组装和微流控技术在内的不同方法进行微加工，以生成具有可控结构的构建体。通过将GelMA与碳纳米管和氧化石墨烯等纳米颗粒以及其他聚合物混合，还可以形成混合水凝胶系统，以形成具有特定生物学应用所需综合性能和特性的网络。最近的研究表明，基于GelMA的水凝胶在广泛的组织工程应用中表现出色，包括骨、软骨、心脏和血管组织工程等。除了组织工程，GelMA水凝胶的其他应用还包括基础细胞研究、细胞信号传导、药物和基因递送以及生物传感。
