肯尼亚乙型肝炎病毒的分子遗传多样性

Molecular genetic diversity of hepatitis B virus in Kenya.

作者信息

Mwangi Joseph, Nganga Zipporah, Songok Elijah, Kinyua Joyceline, Lagat Nancy, Muriuki Joseph, Lihana Raphael, Khamadi Samoel, Osman Saida, Lwembe Raphael, Kiptoo Michael, Mwau Matilu, Chirchir Ruth, Mpoke Solomon, Nyamongo Jack, Okoth Fred, Yamada Rika, Kageyama Seiji, Ichimura Hiroshi

机构信息

Centre for Virus Research, Kenya Medical Research Institute, Nairobi, Kenya.

出版信息

Intervirology. 2008;51(6):417-21. doi: 10.1159/000205526. Epub 2009 Mar 4.

DOI:10.1159/000205526

PMID:19258721

Abstract

Eight genotypes of hepatitis B virus (A-H) and subgenotypes have been recognized worldwide. However, there is limited information on prevalent genotypes in many countries in Africa. This study was undertaken to determine the hepatitis B virus (HBV) genotypes in Kenya. Seropositive HBV blood samples from a blood donor setting were used in the study. HBV genotypes were determined in 52 nucleic acid-positive samples using specific primer in a nested PCR and sequencing employed in the HBV genotyping. This study shows presence of HBV variants with genotypes A (88%), E (8%) and D (4%). In conclusion, we found that HBV genotype A is the most predominant genotype in Kenya with both subgenotype A1 and A2 present. Genotype D and E are also present in our population. This demonstrates that there could be a high genetic diversity of HBV in Kenya.

摘要

全球已识别出八种乙型肝炎病毒基因型（A - H）及其亚基因型。然而，非洲许多国家关于流行基因型的信息有限。本研究旨在确定肯尼亚的乙型肝炎病毒（HBV）基因型。研究使用了来自献血者的血清学阳性HBV血样。通过巢式PCR中的特异性引物和用于HBV基因分型的测序，在52份核酸阳性样本中确定了HBV基因型。本研究显示存在基因型A（88%）、E（8%）和D（4%）的HBV变异体。总之，我们发现HBV基因型A是肯尼亚最主要的基因型，同时存在A1和A2亚基因型。基因型D和E在我们的人群中也有存在。这表明肯尼亚的HBV可能存在高度的遗传多样性。