Department of Neurosciences, School of Dentistry, Section of Prosthodontics and Digital Dentistry, University of Padova, Padova, Italy.
Department of Management and Engineering, University of Padova, Italy.
J Prosthodont Res. 2022 Jul 30;66(3):502-508. doi: 10.2186/jpr.JPR_D_21_00116. Epub 2021 Dec 1.
To compare the flexural properties and the adhesion of Lactobacillus salivarius (LS), Streptococcus mutans (SM), and Candida albicans (CA) on heat-polymerized (CV), CAD-CAM milled (CAD), or 3D-printed (3D) Poly (methylmethacrylate) (PMMA).
Ultimate Flexural Strength (UFS), Flexural Strain (FS) (%) at Flexural Strength, and Flexural Modulus (FM) of specimens (65.0×10.0×3.3 mm) from each PMMA group (n=6) were calculated by using the 3-point bending test. The surface roughness profiles (R) were measured before and after polishing with a contact profilometer. LS, SM, and CA adhesion on PMMA specimens (n=18) (10 mm in diameter, 3 mm in height) was assessed after 90 minutes and 16 hours by using scanning electron microscopy. The Kruskal-Wallis test with post hoc analysis was performed to compare the groups (alpha=0.05).
Mean UFS values were 80.79±7.64 MPa for CV, 110.23±5.03 MPa for CAD, and 87.34±6.39 MPa for 3D. Mean FS values were 4.37±1.04% for CV, 4.71±0.62% for CAD, and 6.19±0.13 % for 3D. Mean FM values were 2542±301 MPa for CV, 3435±346 MPa for CAD, and 2371±197 MPa for 3D. CAD had the lowest average R value (0.29±0.16 µm) before polishing, and bacterial adhesion after 90 minutes of incubation. R value and microbial adhesion were not different amongst groups after polishing and 16 hours of incubation, respectively.
The CAD group displayed the best flexural properties, except for FS, the lowest roughness before polishing and bacterial adhesion after 90 minutes of incubation. All tested PMMAs had similar surface roughness after polishing, and microbial adhesion after 16 hours of incubation.
比较唾液乳杆菌(LS)、变形链球菌(SM)和白色念珠菌(CA)在热聚合(CV)、计算机辅助设计/计算机辅助制造铣削(CAD)和 3D 打印(3D)聚甲基丙烯酸甲酯(PMMA)上的弯曲性能和黏附力。
采用三点弯曲试验计算每组 PMMA 试件(n=6,尺寸为 65.0×10.0×3.3mm)的极限弯曲强度(UFS)、弯曲强度时的弯曲应变(FS)(%)和弯曲模量(FM)。用接触式轮廓仪测量试件表面粗糙度轮廓(R),抛光前后各测量一次。采用扫描电子显微镜(SEM)在 90 分钟和 16 小时后评估 LS、SM 和 CA 在 PMMA 试件(n=18,直径 10mm,高 3mm)上的黏附力。采用 Kruskal-Wallis 检验和事后分析比较各组(α=0.05)。
CV 组的平均 UFS 值为 80.79±7.64MPa,CAD 组为 110.23±5.03MPa,3D 组为 87.34±6.39MPa。CV 组的平均 FS 值为 4.37±1.04%,CAD 组为 4.71±0.62%,3D 组为 6.19±0.13%。CV 组的平均 FM 值为 2542±301MPa,CAD 组为 3435±346MPa,3D 组为 2371±197MPa。CAD 组在抛光前的平均 R 值(0.29±0.16µm)最低,且在孵育 90 分钟后细菌黏附力最低。抛光后和孵育 16 小时后,各组的 R 值和微生物黏附力均无差异。
CAD 组的弯曲性能最好,除 FS 外,抛光前粗糙度最低,孵育 90 分钟后细菌黏附力最低。所有测试的 PMMA 抛光后表面粗糙度相似,孵育 16 小时后微生物黏附力相似。