Fan K, McAlister L
Neurosci Lett. 1985 Aug 16;59(1):111-6. doi: 10.1016/0304-3940(85)90223-x.
Organotypic cultures of newborn rat brains were exposed to the neurotoxin kainic acid or the DNA synthesis inhibitor arabinoside C. The cultures were subsequently co-cultured and the myelination-related enzymatic activities, such as 2',3'-cyclic nucleotide phosphohydrolase and uridine diphosphate-galactose-ceramide galactosyl transferase, were determined under various culture conditions. The newly formed myelin basic protein in the cultured brain tissue was determined by the radioimmunoprecipitation method. The myelination-related enzymatic activities and the synthesis and accumulation of myelin basic protein in the co-cultured brain tissue were found compatible to the control cultures which were not exposed to either drug. The cultures which had been treated with either drug, but not subsequently co-cultured, were found to have decreased enzymatic activities and myelin basic protein synthesis. The experimental data suggest that myelinogenesis requires an interaction between functional neurons and oligodendroglial cells and further supports the hypothesis that the neuron exerts a regulatory effect on the glial myelination mechanism.
将新生大鼠大脑的器官型培养物暴露于神经毒素海人酸或DNA合成抑制剂阿糖胞苷。随后将这些培养物进行共培养，并在各种培养条件下测定与髓鞘形成相关的酶活性，如2',3'-环核苷酸磷酸二酯酶和尿苷二磷酸-半乳糖-神经酰胺半乳糖基转移酶。通过放射免疫沉淀法测定培养脑组织中新形成的髓鞘碱性蛋白。发现在共培养的脑组织中，与髓鞘形成相关的酶活性以及髓鞘碱性蛋白的合成和积累与未暴露于任何一种药物的对照培养物相符。发现用任何一种药物处理但随后未进行共培养的培养物的酶活性和髓鞘碱性蛋白合成降低。实验数据表明，髓鞘形成需要功能性神经元和少突胶质细胞之间的相互作用，并进一步支持神经元对胶质细胞髓鞘形成机制发挥调节作用的假说。
