Chen Xing, Wei Zhirong, Feng Ziqiao, Che Yuhan, Wang Xinyi, Long Hao, Cai Xiaoni, Ren Wei, Xie Zhenyu
School of Marine Biology and Fisheries, Hainan University, Haikou, 570228, China.
Collaborative Innovation Center of Marine Science and Technology, Hainan University, Haikou, 570228, China.
BMC Microbiol. 2025 Mar 8;25(1):125. doi: 10.1186/s12866-025-03837-4.
Lactiplantibacillus pentosus 292 is a lactic acid bacterium (LAB) with significant probiotic potential, but large-scale production is often limited by high production costs and preservation challenges. This study aimed to develop a cost-effective medium to enhance lactic acid production and establish a feasible preservation strategy to support the strain's large-scale application.
A low-cost medium containing glucose, yeast powder, K₂HPO₄, and Tween-80 was formulated, enabling Lactiplantibacillus pentosus 292 to achieve a lactic acid yield of 16.24 g/L, representing an 83.48% increase compared to the traditional MRS medium. Fermentation kinetics models for bacterial growth, substrate consumption, and product generation were established in a 200-L fermenter using the Logistic, Luedeking-Piret-like, and Luedeking-Piret models, and the R values from the model equation were 0.9921 (OD), 0.9942 (dry weight), 0.9506 (total protein), 0.8383 (lactic acid), 0.8898 (total sugar), and 0.8585 (reducing sugar), respectively, indicating that these models were suitable for accurately simulating the growth, nutrient production, and substrate consumption of L. pentosus 292. Additionally, a preservation strategy was developed by using 1-3% molasses as a preservative for the fermentation broth, and its efficacy was verified through temperature acceleration experiments.
In this work, a cost-effective medium that significantly increased lactic acid yield and a preservative based on molasses as a strategy to extend the storage period of fermentation products were developed for large-scale production of L. pentosus 292, a member of probiotic LAB. Additionally, large-scale fermentation kinetics models were constructed, providing valuable technical insights for the large-scale production and application of this LAB, highlighting its significant potential for industrial applications.
戊糖乳杆菌292是一种具有显著益生菌潜力的乳酸菌，但大规模生产常受高生产成本和保存难题的限制。本研究旨在开发一种经济高效的培养基以提高乳酸产量，并建立可行的保存策略以支持该菌株的大规模应用。
配制了一种含葡萄糖、酵母粉、磷酸氢二钾和吐温80的低成本培养基，使戊糖乳杆菌292的乳酸产量达到16.24 g/L，与传统MRS培养基相比提高了83.48%。在200-L发酵罐中使用Logistic、类Luedeking-Piret和Luedeking-Piret模型建立了细菌生长、底物消耗和产物生成的发酵动力学模型，模型方程的R值分别为0.9921（OD）、0.9942（干重）、0.9506（总蛋白）、0.8383（乳酸）、0.8898（总糖）和0.8585（还原糖），表明这些模型适用于准确模拟戊糖乳杆菌292的生长、营养物质产生和底物消耗。此外，开发了一种保存策略，使用1-3%的糖蜜作为发酵液的防腐剂，并通过温度加速实验验证了其有效性。
在本研究中，为益生菌乳酸菌成员戊糖乳杆菌292的大规模生产开发了一种显著提高乳酸产量的经济高效培养基和一种基于糖蜜的防腐剂以延长发酵产物的储存期。此外，构建了大规模发酵动力学模型，为该乳酸菌的大规模生产和应用提供了有价值的技术见解，突出了其在工业应用中的巨大潜力。
