Wang Jianfei, Guo Huanyu, Huang Jiaqi, Jiang Shaoming, Hou Shibo, Chen Xingyu, Lv Hujie, Bi Xudong, Hou Maolin, Lin Hebei, Lu Yuming, Qiao Jinyue, Yang Ruiyi, Liu Shijie
Department of Chemical Engineering, SUNY College of Environmental Science and Forestry, Syracuse, NY13210, USA.
The Center for Biotechnology and Interdisciplinary Studies (CBIS) at Rensselaer Polytechnic Institute, Troy, NY12180, USA.
Bioresour Bioprocess. 2021 Apr 7;8(1):27. doi: 10.1186/s40643-021-00380-8.
Under the optimal conditions of immobilization and fermentation, the highest LA yield of 0.966 ± 0.006 g/g fructose and production rate of 2.426 ± 0.018 g/(L × h) with an error of -0.5% and -0.2% to the predicted results were obtained from batch fermentation by the CS film-coated SA-PVA immobilized L. pentosus cells. The LA yield and production rate of these immobilized cells were 2.7% and 10.1% higher than that of normal SA-PVA immobilized cells respectively, and they were 5.7% and 48.4% higher than that of free cells, respectively. The effect of temperature on different types of immobilized cells and free cells was significantly different, but the effect of pH on different types of cells was not much different. The kinetic models could effectively describe the different fermentation performances of three types of cells. The immobilized cells have excellent reusability to conduct 9 runs of repeated batch fermentation.
在固定化和发酵的最佳条件下，通过CS膜包衣的SA-PVA固定化戊糖乳杆菌细胞进行分批发酵，获得了最高的乳酸产量，为0.966±0.006 g/g果糖，生产率为2.426±0.018 g/(L×h)，与预测结果的误差分别为-0.5%和-0.2%。这些固定化细胞的乳酸产量和生产率分别比普通SA-PVA固定化细胞高2.7%和10.1%，分别比游离细胞高5.7%和48.4%。温度对不同类型的固定化细胞和游离细胞的影响显著不同，但pH对不同类型细胞的影响差异不大。动力学模型可以有效地描述三种类型细胞的不同发酵性能。固定化细胞具有优异的可重复使用性，可进行9次重复分批发酵。
