Bergé-Lefranc J L, Cartouzou G, De Micco C, Fragu P, Lissitzky S
Cancer. 1985 Jul 15;56(2):345-50. doi: 10.1002/1097-0142(19850715)56:2<345::aid-cncr2820560224>3.0.co;2-o.
Thyroglobulin messenger RNA (mRNA) was located and quantified in tissue sections of differentiated human thyroid cancers by in situ hybridization using cloned complementary DNA probes. The cells of the well-differentiated follicular and papillary forms contained similar levels of thyroglobulin mRNA, corresponding to about 2000 copies per cell. In contrast, cells of moderately differentiated thyroid cancers contained about two to three times less thyroglobulin mRNA. It was also found that thyroglobulin mRNA was present almost exclusively in polyribosomes under the form of heavy polyribosomes actively synthesizing thyroglobulin. It is suggested that in situ hybridization method allows localization of specific mRNA in differentiated thyroid cancers and correlation with the level of differentiation of the cells.
通过使用克隆的互补DNA探针进行原位杂交，在分化型人类甲状腺癌的组织切片中定位并定量了甲状腺球蛋白信使核糖核酸（mRNA）。高分化滤泡状和乳头状癌的细胞所含甲状腺球蛋白mRNA水平相似，约为每个细胞2000个拷贝。相比之下，中分化甲状腺癌的细胞所含甲状腺球蛋白mRNA约少两到三倍。还发现甲状腺球蛋白mRNA几乎仅以活跃合成甲状腺球蛋白的重多核糖体形式存在于多核糖体中。提示原位杂交方法可对分化型甲状腺癌中的特定mRNA进行定位，并与细胞分化水平相关联。
