Hamada H, Kohno N, Hiwada K
Second Department of Internal Medicine, Ehime University School of Medicine.
Jpn J Cancer Res. 1994 Feb;85(2):211-9. doi: 10.1111/j.1349-7006.1994.tb02084.x.
KL-6, a circulating mucin-like glycoprotein, is a pulmonary adenocarcinoma-associated antigen and is also regarded as an indicator of disease activity of interstitial pneumonitis. KL-6 has extensive heterogeneous antigenic determinants and consists of multiple heterogeneous antigen molecules. We have searched for circulating KL-6-associated glycoproteins with superior diagnostic value to KL-6 as a tumor marker for pulmonary adenocarcinoma. A new murine monoclonal antibody EH-123 reacting with an asialosugar chain on KL-6 was established. A new KL-6-associated molecule detected by a bimonoclonal bideterminant sandwich assay using the EH-123 antibody as a catcher and horseradish peroxidase-labeled KL-6 as a tracer was designated as CAM 123-6. In 59% (22 of 37) of patients with pulmonary adenocarcinoma, serum levels of CAM 123-6 were abnormally elevated and the positive rate increased with the progression of clinical stage. Elevated levels were not detected in normal individuals or in patients with benign lung diseases, other histologic types of lung cancer, gastric cancer, colon cancer or breast cancer. CAM 123-6 was more specific to pulmonary adenocarcinoma than carcinoembryonic antigen (CEA), but the sensitivity of CAM 123-6 for pulmonary adenocarcinoma was similar to that of CEA. CAM 123-6 is a promising candidate as a serum tumor marker for pulmonary adenocarcinoma.
KL-6是一种循环的黏蛋白样糖蛋白，是肺腺癌相关抗原，也被视为间质性肺炎疾病活动的指标。KL-6具有广泛的异质性抗原决定簇，由多种异质性抗原分子组成。我们一直在寻找对肺腺癌具有比KL-6更高诊断价值的循环KL-6相关糖蛋白作为肿瘤标志物。一种与KL-6上的去唾液酸糖链反应的新型鼠单克隆抗体EH-123被制备出来。使用EH-123抗体作为捕获剂和辣根过氧化物酶标记的KL-6作为示踪剂，通过双单克隆双决定簇夹心测定法检测到的一种新的KL-6相关分子被命名为CAM 123-6。在59%（37例中的22例）肺腺癌患者中，血清CAM 123-6水平异常升高，且阳性率随临床分期进展而增加。在正常个体或患有良性肺部疾病、其他组织学类型肺癌、胃癌、结肠癌或乳腺癌的患者中未检测到水平升高。与癌胚抗原（CEA）相比，CAM 123-6对肺腺癌更具特异性，但CAM 123-6对肺腺癌的敏感性与CEA相似。CAM 123-6有望成为肺腺癌的血清肿瘤标志物候选物。
