Perbeck L, Lund F, Svensson L, Thulin L
Clin Physiol. 1985 Jun;5(3):281-92.
The present work represents an attempt to develop a method for measuring relative blood flow in intestinal capillaries, by the use of sodium fluorescein (Na-F) as an indicator substance. The method is called fluorescein flowmetry (FF). A mathematical model was developed; blood flow was expressed as an index between the maximum fluorescence obtained during the first circulatory passage of Na-F and the rise time, defined as the time interval between 10 and 90% of the maximum fluorescence. The prerequisites for the mathematical model were tested in experiments in animals and man. The extraction of Na-F in the intestinal capillaries was found to be more than 99%. The ratio between the tissue clearance and uptake was found to be 0.0051, indicating no back flux. Experiments in man revealed that the rise time was proportional to the mean transit time and the mean transversal time of the bolus proper. The accuracy of FF was confirmed in man where the ratio between mucosal and serosal-muscular capillary blood flow was 2:1. From the results obtained, it appears that FF can be used as a method for measuring relative capillary blood flow in the intestine.
本研究旨在尝试开发一种利用荧光素钠（Na-F）作为指示物质来测量肠毛细血管相对血流量的方法。该方法称为荧光素血流测定法（FF）。建立了一个数学模型；血流量表示为Na-F首次循环通过期间获得的最大荧光与上升时间之间的指数，上升时间定义为最大荧光的10%至90%之间的时间间隔。在动物和人体实验中对该数学模型的前提条件进行了测试。发现Na-F在肠毛细血管中的提取率超过99%。发现组织清除率与摄取率之比为0.0051，表明无反向通量。人体实验表明，上升时间与推注本身的平均通过时间和平均横向时间成正比。在人体中，粘膜与浆膜-肌层毛细血管血流量之比为2:1，证实了FF的准确性。从获得的结果来看，FF似乎可以用作测量肠道相对毛细血管血流量的方法。
