El-Waziry Islam A K, Eissa Alaa Eldin, El-Tarabili Reham M, Abouelhassan Eman M, Ghetas Hanan A, Ismail Eman M, Elnakeeb Mahmoud A, Abdel Hady Heba A, Aboelnaga Hadeer S, Dessouki Amina A
Department of Aquaculture Diseases Control, Fish Farming and Technology Institute, Suez Canal University, Ismailia 41522, Egypt.
Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
Parasitol Int. 2025 Oct;108:103066. doi: 10.1016/j.parint.2025.103066. Epub 2025 Mar 9.
Parasitic crustaceans affecting cultured marine fish in earthen-pond aquaculture facilities are nasty parasites that trigger cascades of microbial infections with consequent economic losses. In the current study, the role of some parasitic crustaceans in spread and transmission of bacterial infections among cultured marine fishes was investigated utilizing taxonomical, molecular, epidemiological, and pathological assays. A total of 400 marine fish involving Pomadasys stridens, Dicentrarchus labra, Sparus aurata, and Tilapia zilli have been randomly collected from some private earthen pond-based aquaculture facilities. Collected fishes were clinically examined for possible presence of disease symptoms, lesions and/or parasites. Three parasitic crustaceans were identified from gills, skin, and fins of examined marine fish species. The isopod Livoneca redmanii, Caligus, and Lernanthropus species were fully identified. Vibrio alginolyticus was concurrently isolated from both fish and isopod species with 99.76 % similarity between fish and isopod species upon molecular screening utilizing 16SrRNA with sequence alignment. Interestingly, 73.3 % of the isolated bacteria exhibited multi-drug resistance (MDR) with multiple antibiotic resistance (MAR index), values ≥0.2. Further, 56.7 % (17/30) of recovered isolates were resistant to four and five antimicrobials with MAR indices of 0.50 and 0.40. Histopathological examination has revealed remarkable damage of gill filaments and fins of affected fish.
寄生甲壳类动物会影响土池养殖设施中养殖的海水鱼，它们是令人讨厌的寄生虫，会引发一系列微生物感染，从而造成经济损失。在本研究中，利用分类学、分子学、流行病学和病理学检测方法，研究了一些寄生甲壳类动物在养殖海水鱼之间细菌感染传播中的作用。从一些以土池为基础的私人海水养殖设施中随机收集了总共400条海水鱼，包括条纹鸡笼鲳、欧洲鲈鱼、金头鲷和吉利罗非鱼。对收集到的鱼进行临床检查，查看是否存在疾病症状、损伤和/或寄生虫。从检查的海水鱼的鳃、皮肤和鳍上鉴定出三种寄生甲壳类动物。十足目等足类动物雷氏背潜鱼、海鲺属和勒氏鱼虱属均被完全鉴定出来。溶藻弧菌同时从鱼和等足类动物中分离出来，在利用16SrRNA进行分子筛选并进行序列比对时，鱼和等足类动物之间的相似度为99.76%。有趣的是，73.3%的分离细菌表现出多重耐药性（MDR），多重抗生素耐药性（MAR指数）值≥0.2。此外，56.7%（17/30）的回收分离株对四种和五种抗菌药物耐药，MAR指数分别为0.50和0.40。组织病理学检查显示，受影响鱼的鳃丝和鳍受到了明显损伤。
