Hird F J, Robin Y
Comp Biochem Physiol B. 1985;80(3):517-20. doi: 10.1016/0305-0491(85)90283-4.
Mitochondrial preparations from muscles of a crab (Cancer pagurus), two fish (Trachurus trachurus and Scyliorhinus canicula) and a bird (Columba livia) are able to synthesise, through ATP, the phosphagen related to that species. This indicates the presence of a bound phosphagen kinase. Addition of creatine kinase and creatine to crab mitochondria results in the synthesis of phosphocreatine. Similarly, the addition of arginine kinase and arginine to mitochondrial preparations from the fish and bird results in the synthesis of phosphoarginine. In the crab, the mitochondrial form of arginine kinase released by sonication had the same kinetic affinity constants and electrophoretic mobility and could not be distinguished immunologically from the cytosolic form. The close similarity of bound and cytosolic forms of arginine kinase in this crustacean suggests that the two forms have not evolved separately as has creatine kinase in the mammal.
来自一只螃蟹（黄道蟹）、两种鱼类（竹荚鱼和小斑猫鲨）以及一只鸟类（家鸽）肌肉的线粒体提取物能够通过ATP合成与该物种相关的磷酸原。这表明存在一种结合型磷酸原激酶。向螃蟹线粒体中添加肌酸激酶和肌酸会导致磷酸肌酸的合成。同样，向鱼类和鸟类的线粒体提取物中添加精氨酸激酶和精氨酸会导致磷酸精氨酸的合成。在螃蟹中，通过超声处理释放的线粒体形式的精氨酸激酶具有相同的动力学亲和常数和电泳迁移率，并且在免疫上无法与胞质形式区分开来。这种甲壳类动物中结合型和胞质型精氨酸激酶的高度相似性表明，这两种形式并没有像哺乳动物中的肌酸激酶那样独立进化。
