Xu Xin, Wang Ruotong, Pei Linlin, Wang Quanfeng, Liu Chunjie
College of Life Science and Technology, State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, Tarim University, Alar, China.
College of Animal Science, Key Laboratory of Livestock and Forage Resources Utilization Around Tarim, Ministry of Agriculture and Rural Affairs, Tarim University, Alar, China.
Vet Med Sci. 2025 Mar;11(2):e70294. doi: 10.1002/vms3.70294.
Glycolysis in granulosa cells (GCs) is the primary location of energy metabolism and its substrates in oocytes and is closely related to follicular development in mammals. The complex morphological structure and physiological functions of GCs are regulated by follicle-stimulating hormone (FSH), but little is known about how FSH regulates glycolysis in GCs, and its mechanism remains unclear. The purpose of this study is to investigate the mechanism by which FSH activates the Akt/FOXO1 pathway, thereby regulating glucose metabolism in ovine GCs. Granulosa cells were cultured in the presence of different concentrations of FSH and a CCK-8 assay was used to measure the proliferation of the treated cells. Next, qRT-PCR was performed to measure the transcription of the target genes, glucose transporter (GLUT). Western-blot analysis of the phospho-Akt and -FOXO1 levels induced by FSH through the glucose signalling pathway, in addition to the effects of these proteins on the expression levels of the downstream GLUT genes, was measured. Results showed that the addition of 10ng/mL FSH to the culture medium increased the viability of granulosa cells. Transcription of GLUT1 and 4 was significantly up-regulated in FSH-treated cells through activation of the Akt/FOXO1 phosphorylation pathway, thereby affecting glucose metabolism. This study contributes to the current understanding of the metabolic features of and the associated developmental pathways of ovine follicular GCs.
颗粒细胞（GCs）中的糖酵解是卵母细胞能量代谢及其底物的主要场所，与哺乳动物的卵泡发育密切相关。GCs复杂的形态结构和生理功能受促卵泡激素（FSH）调节，但FSH如何调节GCs中的糖酵解及其机制尚不清楚。本研究的目的是探讨FSH激活Akt/FOXO1途径从而调节绵羊GCs葡萄糖代谢的机制。在不同浓度的FSH存在下培养颗粒细胞，并使用CCK-8测定法测量处理后细胞的增殖。接下来，进行qRT-PCR以测量靶基因葡萄糖转运蛋白（GLUT）的转录。通过葡萄糖信号通路对FSH诱导的磷酸化Akt和-FOXO1水平进行蛋白质免疫印迹分析，此外还测量了这些蛋白质对下游GLUT基因表达水平的影响。结果表明，向培养基中添加10ng/mL FSH可提高颗粒细胞的活力。通过激活Akt/FOXO1磷酸化途径，FSH处理的细胞中GLUT1和4的转录显著上调，从而影响葡萄糖代谢。本研究有助于当前对绵羊卵泡GCs的代谢特征及其相关发育途径的理解。
