Dehm E J, Puliafito C A, Adler C M, Steinert R F
Arch Ophthalmol. 1986 Sep;104(9):1364-8. doi: 10.1001/archopht.1986.01050210118037.
We performed a morphologic investigation of the corneal endothelium following in vivo excimer laser ablation at 193 and 248 nm in rabbit eyes. Control experiments were performed in eyes with incisions of similar depth made with a diamond surgical knife, and normal control eyes were studied in parallel. We found that 193-nm excimer laser incision to 90% of corneal depth produces endothelial alterations similar to those seen underlying diamond knife incisions of similar depth. Scanning electron microscopy revealed an incisional ridge and endothelial cellular edema; endothelial cell loss was not observed. In contrast, 248-nm ablations of similar depth and energy density revealed loss of underlying endothelial cells and a surrounding zone of severe cellular damage.
我们对兔眼进行了193纳米和248纳米准分子激光体内消融后角膜内皮的形态学研究。用钻石手术刀制作了相似深度切口的眼睛作为对照实验，并同时研究了正常对照眼。我们发现，193纳米准分子激光切口至角膜深度的90%会产生与相似深度钻石刀切口下方所见相似的内皮改变。扫描电子显微镜显示有切口嵴和内皮细胞水肿；未观察到内皮细胞丢失。相比之下，相似深度和能量密度的248纳米消融显示下方内皮细胞丢失以及周围严重细胞损伤区域。
