Zhu Gege, Li Xinyu, Ma Qianjie, Hong Zhanying, Zhong Dan, Sun Xiaodong
Shanghai Engineering Research Center of Organ Repair, Joint International Research Laboratory of Biomaterials and Biotechnology in Organ Repair (Ministry of Education), School of Medicine, Shanghai University, Shanghai, 200444, China.
School of Pharmacy, Second Military Medical University, Shanghai, 200433, China.
Mikrochim Acta. 2025 Mar 11;192(4):220. doi: 10.1007/s00604-025-07058-4.
The 5-Hydroxytryptamine (5-HT) level determination is crucial for predicting, pathogenesis, diagnosis, and pharmacological treatment of schizophrenia. To realize the extraction of trace 5-HT in complex matrix samples, a magnetic molecularly imprinted solid-phase extraction (MMISPE) pretreatment method was developed. In combination with UPLC-MS/MS, the method was possible to achieve the quantification of 1 ng/mL 5-HT in tissue samples. The type of adsorbent, magnetic surface molecularly imprinted polymers (MMIPs) adsorbent dosage, adsorption temperature, and adsorption method were screened to obtain the better extraction of 5-HT. After optimizing the extraction and separation method, we applied the MMISPE method to the detection of 5-HT in peripheral blood of clinical schizophrenia patients. The results showed that the average concentration of 5-HT in the peripheral blood of healthy controls was 74.30 ng/mL, whereas the 5-HT content in the samples from schizophrenic patients was 1.17 ng/mL.
5-羟色胺（5-HT）水平测定对于精神分裂症的预测、发病机制、诊断及药物治疗至关重要。为实现复杂基质样品中痕量5-HT的提取，开发了一种磁性分子印迹固相萃取（MMISPE）预处理方法。结合超高效液相色谱-串联质谱（UPLC-MS/MS），该方法能够实现对组织样品中1 ng/mL 5-HT的定量分析。对吸附剂类型、磁性表面分子印迹聚合物（MMIPs）吸附剂用量、吸附温度及吸附方法进行筛选，以实现对5-HT的更佳提取。优化提取和分离方法后，将MMISPE方法应用于临床精神分裂症患者外周血中5-HT的检测。结果显示，健康对照者外周血中5-HT的平均浓度为74.30 ng/mL，而精神分裂症患者样品中5-HT含量为1.17 ng/mL。
