Gao Song, Li Fu, Zeng Zheng, He Qiaoyun, Mostafa Hassan H A, Zhang Suling, Wang Taotao, Wang Yanzhou, Liu Touming
Key Laboratory of Biobreeding for Specialty Horticultural Crops of Jiangsu Province, College of Horticulture and Landscape Architecture, Yangzhou University, No. 88, Southern road of Daxue, 225009, Yangzhou, China.
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, No. 348, Western road of Xiajiahu, 410205, Changsha, China.
Hortic Res. 2025 Jan 3;12(4):uhae365. doi: 10.1093/hr/uhae365. eCollection 2025 Apr.
The garlic bulb comprises several cloves, the swelling growth of which is significantly hindered by the accumulation of viruses. Herein, we describe a single-cell transcriptomic atlas of swelling cloves with virus accumulation, which comprised 19 681 high-quality cells representing 11 distinct cell clusters. Cells of two clusters, clusters 7 (C7) and 11 (C11), were inferred to be from the meristem. Cell trajectory analysis suggested the differentiation of clove cells to start from the meristem cells, along two pseudo-time paths. Investigation into the cell-specific activity of invasive viruses demonstrated that garlic virus genes showed relatively low-expression activity in cells of the clove meristem. There were 2060 garlic genes co-expressed with virus genes, many of which showed an association with the defense response. Five glutathione synthase/reductase genes co-expressed with virus genes displayed up-regulated expression, and the glutathione and related metabolites level showed an alteration in virus-invasive garlic clove, implying the role of glutathione in viral immunity of garlic. Our study offers valuable insights into the clove organogenesis and interaction between garlic and virus at single-cell resolution.
大蒜鳞茎由多个蒜瓣组成，病毒的积累会显著阻碍蒜瓣的膨大生长。在此，我们描述了一个具有病毒积累的膨大蒜瓣的单细胞转录组图谱，该图谱包含19681个高质量细胞，代表11个不同的细胞簇。推断7号簇（C7）和11号簇（C11）这两个簇的细胞来自分生组织。细胞轨迹分析表明，蒜瓣细胞的分化从分生组织细胞开始，沿着两条拟时间路径进行。对侵袭性病毒的细胞特异性活性的研究表明，大蒜病毒基因在蒜瓣分生组织细胞中的表达活性相对较低。有2060个大蒜基因与病毒基因共表达，其中许多基因与防御反应有关。与病毒基因共表达的5个谷胱甘肽合酶/还原酶基因表现出上调表达，并且在病毒侵袭的大蒜蒜瓣中谷胱甘肽及相关代谢物水平发生了改变，这意味着谷胱甘肽在大蒜的病毒免疫中发挥作用。我们的研究在单细胞分辨率下为蒜瓣器官发生以及大蒜与病毒之间的相互作用提供了有价值的见解。
