Bowersox J C, Sorgente N
Diabetes. 1985 Jul;34(7):628-33. doi: 10.2337/diab.34.7.628.
We cultured arterial smooth muscle cells and dermal fibroblasts from spontaneously diabetic BB rats and normoglycemic littermate controls. Although there were no detectable differences in the morphology of cells obtained from diabetic rats, significant differences existed in growth parameters of the diabetic smooth muscle cells. These cells grew more rapidly than smooth muscle cells from normal rats (population doubling times: normal = 42.6 +/- 3.2 h, diabetic = 31.8 +/- 3.7 h) and attained greater densities at confluence. The growth rates of the diabetic smooth muscle cells were dependent on the initial seeding density of cells, a characteristic not observed in cells from normal rats. Although growth rates of the diabetic smooth muscle cells were increased, their plating efficiencies were reduced. Dermal fibroblasts from diabetic rats grew at the same rates as fibroblasts from control animals and the plating efficiencies of the diabetic fibroblasts were increased rather than reduced. In these studies, we have shown that vascular-derived cells from diabetic rats have growth defects not detected in dermal fibroblasts from the same animals. This emphasizes the importance of using blood vessel cells to probe the pathophysiology of diabetic vascular disease. Furthermore, our results establish the validity of using spontaneously diabetic rats as a model system for examining inherent cell defects in insulin-dependent diabetes mellitus.
我们从自发性糖尿病BB大鼠和血糖正常的同窝对照大鼠中培养动脉平滑肌细胞和真皮成纤维细胞。尽管从糖尿病大鼠获得的细胞在形态上没有可检测到的差异，但糖尿病平滑肌细胞的生长参数存在显著差异。这些细胞比正常大鼠的平滑肌细胞生长得更快（群体倍增时间：正常 = 42.6 ± 3.2小时，糖尿病 = 31.8 ± 3.7小时），并且汇合时达到更高的密度。糖尿病平滑肌细胞的生长速率取决于细胞的初始接种密度，这一特征在正常大鼠的细胞中未观察到。尽管糖尿病平滑肌细胞的生长速率增加，但其接种效率降低。糖尿病大鼠的真皮成纤维细胞与对照动物的成纤维细胞生长速率相同，并且糖尿病成纤维细胞的接种效率增加而非降低。在这些研究中，我们表明糖尿病大鼠的血管来源细胞存在生长缺陷，而在同一动物的真皮成纤维细胞中未检测到。这强调了使用血管细胞来探究糖尿病血管疾病病理生理学的重要性。此外，我们的结果确立了使用自发性糖尿病大鼠作为模型系统来研究胰岛素依赖型糖尿病固有细胞缺陷的有效性。
