Dalla Costa Vanessa, Piovan Anna, Varfaj Ina, Marcotullio Maria Carla, Brun Paola, Filippini Raffaella
Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo, 5, 35131 Padua, Italy.
Department of Pharmaceutical Sciences, University of Perugia, Via Fabretti 48, 06123 Perugia, Italy.
Molecules. 2025 Feb 27;30(5):1089. doi: 10.3390/molecules30051089.
var. Marasca (Rosaceae) is an important Croatian cultivar, known wordwide for the production of Luxardo maraschino liqueur, which occurs in the eastern Po Valley of Italy. Besides liqueur, Marasca is attractive for its beneficial effects on human health and well-being. The undifferentiated in vitro cell cultures of Marasca were investigated as a source of healthy products. The in vitro conditions for obtaining callus and suspension cultures under photoperiod were defined. The cell lines were evaluated for growth rate, total phenol and proanthocyanidin contents, and antioxidant activities via colorimetric assays. The best cell lines were also subcultured in darkness, studying the importance of the light parameter in the possible industrial scaling-up. The juices extracted from the obtained biomasses were analyzed by LC-DAD-MS and six flavanone derivatives, among which naringenin and its glucoside were identified. The quantitative analysis, pursued during the cell growth cycle, revealed that the flavanone content was higher at the end of the growth cycle (28th day) and that the total content of identified flavanone compounds varied from 17.22 to 79.22 μg/mL of juice. The results of the antioxidant and anti-inflammatory activities on Caco-2 cells support the potential applications of this material in human health.
马拉斯卡变种（蔷薇科）是克罗地亚的一个重要品种，以生产卢萨多樱桃利口酒而闻名于世，该品种生长于意大利波河河谷东部。除了利口酒，马拉斯卡因其对人类健康和福祉的有益影响而备受关注。对马拉斯卡的未分化体外细胞培养物作为健康产品来源进行了研究。确定了在光周期条件下获得愈伤组织和悬浮培养物的体外条件。通过比色法评估细胞系的生长速率、总酚和原花青素含量以及抗氧化活性。还将最佳细胞系在黑暗中进行继代培养，研究光参数在可能的工业放大过程中的重要性。对从获得的生物质中提取的汁液进行了LC-DAD-MS分析，鉴定出六种黄烷酮衍生物，其中包括柚皮素及其糖苷。在细胞生长周期中进行的定量分析表明，黄烷酮含量在生长周期结束时（第28天）较高，已鉴定的黄烷酮化合物总含量在每毫升汁液17.22至79.22微克之间。对Caco-2细胞的抗氧化和抗炎活性研究结果支持了这种材料在人类健康方面的潜在应用。
