Del Sole Roberta, Montefusco Maria Assunta, Filippini Raffaella, Mergola Lucia
Department of Engineering for Innovation, University of Salento, Via per Monteroni Km 1, 73100 Lecce, Italy.
Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, Italy.
Antioxidants (Basel). 2024 Jun 25;13(7):765. doi: 10.3390/antiox13070765.
is a genus of wild perennial shrubs native in an area extending from Southwest Europe to Southeast Asia, and it is used in traditional medicine for the diuretic, cardiac, and tonic properties of its fruits, which can also be cooked to make jellies, jams, and sauces. This work aims to study and compare the antioxidant activity and the phenolic and anthocyanin composition of three varieties of : Red Column (PCR), Orange Glow (PCO), and Soleil d'Or (PCS), and one of : Orange glow (PAO), collected from the spontaneous flora of the Mediterranean region (Southern Italy). Two different extraction processes were tested using methanol and an aqueous methanol solution (80% MeOH) to evaluate the polyphenolic content and antioxidant activity of freeze-dried berries. The highest total phenolic content was found in PCR and PAO berries (174.21 ± 0.149 and 168.01 ± 0.691 mg of gallic acid equivalent per gram of dry matter, respectively) extracted with an aqueous methanol solution (80% MeOH). Polyphenolic extracts analyzed by HPLC-DAD-ESI/MS confirmed the presence of rutin, quercetin hexose, neoeriocitrin, procyanidin B, and resveratrol. Moreover, the total antioxidant activity of the berries' extracts was measured by comparing two different spectrophotometric methods (ABTS and DPPH), showing that the varieties with the highest total phenolic content, PCR and PAO, also had the highest scavenging activity. Finally, a suitable extraction process was chosen for the evaluation of the anthocyanins' composition of all frozen berries, and in all MS spectra of varieties, two ionic species at 449 m/z attributable to two cyanidin derivatives were found.
是一种野生多年生灌木属，原产于从西南欧到东南亚的地区，其果实具有利尿、强心和滋补特性，可用于传统医学，果实还可烹饪制成果冻、果酱和调味汁。本研究旨在研究和比较三种 品种：红柱（PCR）、橙辉（PCO）和金色阳光（PCS），以及一种 品种：橙辉（PAO）的抗氧化活性、酚类和花青素成分，这些品种采自地中海地区（意大利南部）的野生植物群。使用甲醇和甲醇水溶液（80%甲醇）测试了两种不同的提取工艺，以评估冷冻干燥浆果的多酚含量和抗氧化活性。用甲醇水溶液（80%甲醇）提取的PCR和PAO浆果中总酚含量最高（分别为每克干物质174.21±0.149和168.01±0.691毫克没食子酸当量）。通过HPLC-DAD-ESI/MS分析的多酚提取物证实了芦丁、槲皮素己糖、新橙皮苷、原花青素B和白藜芦醇的存在。此外，通过比较两种不同的分光光度法（ABTS和DPPH）测量了浆果提取物的总抗氧化活性，结果表明总酚含量最高的品种PCR和PAO也具有最高的清除活性。最后，选择了一种合适的提取工艺来评估所有冷冻浆果的花青素组成，在所有 品种的质谱图中，发现了两种归因于两种矢车菊素衍生物的449 m/z离子物种。
