Wang Dongsheng, Cheng Beibei, Yu Liyang, Yuan Guomei, Ma Yate, Zhang Jijun, Lin Furong
Hebei Province Yanshan Agriculture Characteristic Industry Technology Research Institute, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China.
Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization, Hebei Normal University of Science and Technology, Qinhuangdao 066600, China.
Molecules. 2025 Mar 3;30(5):1149. doi: 10.3390/molecules30051149.
Anthocyanins constitute the primary pigment components in hawthorn () peel, yet their specific composition and concentration profiles remain poorly characterized. This study employed ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS)-based metabolomics to systematically compare anthocyanin profiles between red-peel (CPR) and yellow-peel (CPY) hawthorn cultivars. Our analysis identified 26 anthocyanin metabolites in CPR and 24 in CPY, with cyanidin-3-O-galactoside and cyanidin-3-O-arabinoside being the predominant compounds in both. Multivariate analysis revealed seven significantly differential metabolites, including cyanidin-3-O-galactoside, cyanidin-3-O-arabinoside, pelargonidin-3-O-galactoside, pelargonidin-3-O-glucoside, pelargonidin-3-O-arabinoside, and peonidin-3-O-galactoside. Notably, all the differential metabolites exhibited reductions in CPY compared to CPR. Chromatic analysis demonstrated that CPR possessed highly significantly lower hue angle values (h) than CPY (47.7093 ± 4.1706, 83.6427 ± 1.4604, < 0.01), showing strong negative correlations with key anthocyanins. These findings enhance the scientific understanding of anthocyanin biosynthesis in hawthorn peel and provide a certain reference for the development and utilization of anthocyanins in hawthorn peel.
花色苷是山楂果皮中的主要色素成分，但其具体组成和浓度分布仍不清楚。本研究采用基于超高效液相色谱-电喷雾电离-串联质谱（UPLC-ESI-MS/MS）的代谢组学方法，系统比较了红皮山楂（CPR）和黄皮山楂（CPY）品种之间的花色苷谱。我们的分析在CPR中鉴定出26种花色苷代谢物，在CPY中鉴定出24种，其中矢车菊素-3-O-半乳糖苷和矢车菊素-3-O-阿拉伯糖苷是两者中的主要化合物。多变量分析揭示了7种显著差异的代谢物，包括矢车菊素-3-O-半乳糖苷、矢车菊素-3-O-阿拉伯糖苷、天竺葵素-3-O-半乳糖苷、天竺葵素-3-O-葡萄糖苷、天竺葵素-3-O-阿拉伯糖苷和芍药素-3-O-半乳糖苷。值得注意的是，与CPR相比，所有差异代谢物在CPY中均呈现减少趋势。色度分析表明，CPR的色相角值（h）显著低于CPY（47.7093±4.1706，83.6427±1.4604，P<0.01），与关键花色苷呈强负相关。这些发现加深了对山楂果皮花色苷生物合成的科学理解，并为山楂果皮花色苷的开发利用提供了一定参考。
