Li Mingrui, Weng Zhuo, Gong Zihan, Li Xiaoyu, Ye Jiayi, Gao Yufu, Rong Liping
College of Agriculture, Yanbian University, Yanji 133000, China.
Int J Mol Sci. 2025 Feb 21;26(5):1865. doi: 10.3390/ijms26051865.
Anthocyanin synthetase (ANS), a key enzyme in the final step of the anthocyanin synthesis pathway, catalyzes the conversion of leucoanthocyanidins to anthocyanins. In this study, an ANS structural protein (TRINITY_DN18024_c0_g1) was found to be associated with anthocyanin accumulation in leaves, named . Real-time quantitative fluorescence PCR analysis revealed that the expression of was significantly higher in red-leaved (variant) than green-leaved (wild-type) strains, which was consistent with the transcriptome data. The UPLC results showed that the cyanidin metabolites may be the key substance influencing the final color formation of . The gene was cloned and analyzed through bioinformatics analysis. has a total length of 1371 bp, and it encodes 360 amino acids. Analysis of the structural domain of the protein revealed that contains a PcbC functional domain. Protein secondary structure predictions indicate that α-helix, irregularly coiled, and extended chains are the major building blocks. Subcellular localization predicted that might be localized in the nucleus. The phylogenetic tree revealed that is relatively closely related to in . The prediction of miRNA showed that the gene is regulated by miR6200. This study provides a theoretical reference for further analyzing the regulatory mechanism of leaf color formation in .
花青素合成酶（ANS）是花青素合成途径最后一步的关键酶，催化无色花青素向花青素的转化。在本研究中，发现一种ANS结构蛋白（TRINITY_DN18024_c0_g1）与叶片中的花青素积累相关，命名为 。实时定量荧光PCR分析表明，红叶（变种）菌株中 的表达明显高于绿叶（野生型）菌株，这与转录组数据一致。超高效液相色谱结果表明，矢车菊素代谢产物可能是影响 最终颜色形成的关键物质。通过生物信息学分析对 基因进行了克隆和分析。 全长1371 bp，编码360个氨基酸。对 蛋白结构域的分析表明， 含有一个PcbC功能域。蛋白质二级结构预测表明，α-螺旋、不规则卷曲和延伸链是主要组成部分。亚细胞定位预测 可能定位于细胞核。系统发育树显示， 与 中的 关系相对密切。miRNA预测表明， 基因受miR6200调控。本研究为进一步分析 叶片颜色形成的调控机制提供了理论参考。
