Guo Caifang, Zhang Yi, Bai Dongying, Zhen Wenrui, Ma Penghui, Wang Ziwei, Zhao Xiaodie, Ma Xiqiang, Xie Xiaolin, Ito Koichi, Zhang Bingkun, Yang Yajun, Li Jianyong, Ma Yanbo
Department of Animal Physiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China.
Henan International Joint Laboratory of Animal Welfare and Health Breeding, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.
Int J Mol Sci. 2025 Feb 21;26(5):1877. doi: 10.3390/ijms26051877.
This study aimed to evaluate the effects of aspirin eugenol ester (AEE) on growth performance, oxidative liver damage, inflammation, and liver metabolomics in broilers under high-stocking-density (HSD) stress. A total of 360 broilers were divided into four groups: normal density (ND, 14/m), high density (HD, 22/m), ND-AEE (ND + 0.01% AEE), and HD-AEE (HD + 0.01% AEE). HSD decreased total antioxidant capacity, increased malondialdehyde (MDA) levels, and elevated the expression of cyclooxygenase-2 () and microsomal prostaglandin E synthase-1 () mRNA, which contributed to the reduced performance of broilers. Specifically, HSD caused abnormalities in linoleic acid metabolism, leading to elevated levels of Prostaglandin E2 (PGE2) and Leukotriene B4 (LTB4) synthesis, which aggravated inflammation, increased liver lipid levels, and impaired ATP production. AEE counteracted the decline in broiler production performance induced by HSD by enhancing total antioxidant capacity, reducing MDA levels, protecting the liver from oxidative damage, and maintaining mitochondrial oxidative phosphorylation. AEE positively regulated the linoleic acid metabolism by promoting the synthesis of γ-linolenic acid and phosphatidylcholine, which reduced the synthesis of and . AEE alleviated the metabolic imbalance caused by HSD stress and enhanced the efficiency of mitochondrial fatty acid oxidation, which reduced excess lipid accumulation in the liver and promoted ATP production. In summary, this study provides strong support for the dietary addition of AEE to alleviate liver oxidative damage, inflammation, and energy metabolism disorders caused by HSD stress.
本研究旨在评估阿司匹林丁香酚酯（AEE）对高饲养密度（HSD）应激下肉鸡生长性能、肝脏氧化损伤、炎症及肝脏代谢组学的影响。总共360只肉鸡被分为四组：正常密度（ND，14只/平方米）、高密度（HD，22只/平方米）、ND - AEE（ND + 0.01% AEE）和HD - AEE（HD + 0.01% AEE）。HSD降低了总抗氧化能力，增加了丙二醛（MDA）水平，并提高了环氧化酶 - 2（COX - 2）和微粒体前列腺素E合酶 - 1（mPGES - 1）mRNA的表达，这导致了肉鸡生产性能下降。具体而言，HSD导致亚油酸代谢异常，导致前列腺素E2（PGE2）和白三烯B4（LTB4）合成水平升高，这加剧了炎症，增加了肝脏脂质水平，并损害了ATP生成。AEE通过增强总抗氧化能力、降低MDA水平、保护肝脏免受氧化损伤以及维持线粒体氧化磷酸化，抵消了HSD诱导的肉鸡生产性能下降。AEE通过促进γ - 亚麻酸和磷脂酰胆碱的合成来正向调节亚油酸代谢，从而减少了PGE2和LTB4的合成。AEE减轻了HSD应激引起的代谢失衡，并提高了线粒体脂肪酸氧化效率，从而减少了肝脏中多余的脂质积累并促进了ATP生成。总之，本研究为日粮添加AEE以减轻HSD应激引起的肝脏氧化损伤、炎症和能量代谢紊乱提供了有力支持。
