使用等电聚焦（IEF）作为红细胞酸性磷酸酶（EAP）和酯酶D（EsD）联合表型分析的一种方法。

The use of isoelectric focusing (IEF) as a method for the combined phenotyping of erythrocyte acid phosphatase (EAP) and esterase D (EsD).

作者信息

Finney S T, Renshaw N A, Werrett D J

出版信息

Forensic Sci Int. 1985 Apr;27(4):237-45. doi: 10.1016/0379-0738(85)90143-4.

Abstract

The simultaneous isoelectric focusing (IEF) in polyacrylamide gels (PAG) of erythrocyte acid phosphatase (EAP) and esterase D (EsD) allows the poor discriminating power (DP) of EsD to be usefully combined with a highly discriminating system EAP, such that a joint DP of 0.766 was achieved compared with PGM IEF DP 0.756. Focusing was carried out in a centrally flattened gradient containing ampholines (pH 4-6 and 6-8) and the chemical spacer 3-(N-morpholino) propanesulphinic acid (MOPS). It enabled the identification of six EsD phenotypes including the recently discovered EsD5 isozymes. The application of this method to casework bloodstains is discussed.

摘要

在聚丙烯酰胺凝胶（PAG）中对红细胞酸性磷酸酶（EAP）和酯酶D（EsD）进行同步等电聚焦（IEF），可将鉴别力较差的EsD与鉴别力高的EAP系统有效结合，从而实现联合鉴别力为0.766，而磷酸葡萄糖变位酶IEF鉴别力为0.756。聚焦在含有两性电解质（pH 4 - 6和6 - 8）和化学间隔物3 -（N - 吗啉代）丙烷磺酸（MOPS）的中心扁平梯度中进行。该方法能够鉴定出六种EsD表型，包括最近发现的EsD5同工酶。本文还讨论了该方法在实际案件血迹中的应用。