Tamaki Y, Nishimukai H, Kishida T, Fukuda M
Department of Forensic Medicine, Medical College of Oita, Japan.
Z Rechtsmed. 1987;99(2):135-42. doi: 10.1007/BF00200633.
Time-and cost-saving methods for paternity testing are described. Seventeen genetic systems were divided into six groups: (1) transferrin (Tf), factor B (Bf), and phosphoglucomutase 1 (PGM1); (2) group-specific component (Gc) or alpha 1-antitrypsin (PI) and alpha 2HS-glycoprotein (HSGA); (3) complement components C6 and C7, factor 13B (F13B), and plasminogen (PLG); (4) haptoglobin (Hp), C8 alpha-gamma chain (C81), and factor I (IF); (5) red cell acid phosphatase (ACP), esterase D (ESD), and glutamic-pyruvic transaminase (GPT); and (6) 6-phosphogluconate dehydrogenase (PGD) and glyoxalase I (GLO). Each group of systems was typed simultaneously by electrophoresis or isoelectric focusing (IEF) followed by staining or immunoblotting. These methods are very practical because they afford a considerable saving of time, work and expense, and facilitate semipermanent preservation of electrophoretic patterns.
本文描述了节省亲子鉴定时间和成本的方法。17个遗传系统被分为6组：(1)转铁蛋白(Tf)、B因子(Bf)和磷酸葡萄糖变位酶1(PGM1)；(2)组特异性成分(Gc)或α1-抗胰蛋白酶(PI)和α2HS-糖蛋白(HSGA)；(3)补体成分C6和C7、因子13B(F13B)和纤溶酶原(PLG)；(4)触珠蛋白(Hp)、C8α-γ链(C81)和因子I(IF)；(5)红细胞酸性磷酸酶(ACP)、酯酶D(ESD)和谷丙转氨酶(GPT)；以及(6)6-磷酸葡萄糖酸脱氢酶(PGD)和乙二醛酶I(GLO)。每组系统通过电泳或等电聚焦(IEF)，然后进行染色或免疫印迹同时进行分型。这些方法非常实用，因为它们可节省大量时间、工作量和费用，并便于电泳图谱的半永久性保存。
