Pozo Maria R, Heinson Yuli W, Chua Christianne J, Entcheva Emilia
Department of Biomedical Engineering, George Washington University, Washington, District of Columbia.
Department of Biomedical Engineering, George Washington University, Washington, District of Columbia.
Biophys J. 2025 Apr 15;124(8):1273-1284. doi: 10.1016/j.bpj.2025.03.006. Epub 2025 Mar 12.
The Halbach array, originally developed for particle accelerators, is a compact arrangement of permanent magnets that creates well-defined magnetic fields without heating. Here, we demonstrate its use for modulating the speed of electromechanical waves in cardiac syncytia of human stem cell-derived cardiomyocytes. At 40-50 mT magnetic field strength, a cylindrical dipolar Halbach array boosted the conduction velocity (CV) by up to 25% when the magnetic field was co-aligned with the electromechanical wave (but not when perpendicular to it). To observe the effects, a short-term incubation of the cardiac cell constructs with non-targeted magnetic nanoparticles (mNPs) was sufficient. This led to increased CV anisotropy, and effects were most pronounced at slower pacing rates. Instantaneous formation and rearrangement of elongated mNP clusters upon magnetic-field rotation was seen, creating dynamic structural anisotropy that may have contributed to the directional CV effects. This approach may be useful for anti-arrhythmic control of cardiac waves.
哈尔巴赫阵列最初是为粒子加速器开发的，它是一种紧凑的永久磁体排列方式，能够在不产生热量的情况下产生明确的磁场。在此，我们展示了其用于调节人干细胞衍生心肌细胞心脏合胞体中机电波速度的用途。在40 - 50毫特斯拉的磁场强度下，当磁场与机电波共线时（但垂直时则不然），圆柱形偶极哈尔巴赫阵列可使传导速度（CV）提高多达25%。为了观察这些效果，将心脏细胞构建体与非靶向磁性纳米颗粒（mNP）进行短期孵育就足够了。这导致CV各向异性增加，且在较慢的起搏频率下效果最为明显。在磁场旋转时可观察到细长的mNP簇瞬间形成和重新排列，产生动态结构各向异性，这可能是导致CV方向效应的原因。这种方法可能有助于对心脏波进行抗心律失常控制。
