Mediates Immunosuppression of the Tumor Microenvironment in Non-Small Cell Lung Cancer.

BACKGROUND

Studies have demonstrated that histone deacetylase 1 () enables cancer cells to evade killing mediated by cytotoxic T lymphocytes. However, there are no studies on the immunological aspects of in non-small cell lung cancer (NSCLC).

METHODS

In this research, we used the Cancer Genome Atlas (TCGA) public database combined with tissue microarray (TMA) to investigate expression and prognosis in NSCLC. According to the median value of expression in the TCGA dataset, samples of patients with NSCLC were classified into high- and low-expression cohorts. Subsequently, the biological characteristics of in high- and low-expression cohorts in terms of signaling pathways, immune cell infiltration, immune cell function, and genomic stability were investigated to better understand the effect of in the tumor microenvironment (TME) of NSCLC. Additionally, we selected tissue samples with overexpression in TMA2 and performed immunohistochemical staining of CD8 T cells to observe the distribution of CD8 T cells in the tumor.

RESULTS

The findings revealed that overexpression of in NSCLC was associated with poor prognosis. Analysis of signaling pathway enrichment indicated that downregulated immune-related signaling pathways in NSCLC. Immune cell infiltration, immune cell function, and genomic stability analyses suggested that the TME alteration mediated by in the high-expression cohort was consistent with the "immune desert" phenotype. Furthermore, CD8 T immunohistochemical staining experiments of tissue samples with overexpression in NSCLC revealed few CD8 T cells distributed in the tumor parenchyma and interstitium.

CONCLUSION

Conclusively, our findings from several biological analyses revealed that is overexpressed in NSCLC and induces TME immunosuppression.