An Jiangru, Ma Tianyi, Wang Qiuhua, Zhang Jinyi, Santerre J Paul, Wang Wenshuang, Ma Peng, Zhang Xiaoqing
International Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University, Yantai, Shandong, China.
Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.
Front Bioeng Biotechnol. 2025 Feb 26;13:1551791. doi: 10.3389/fbioe.2025.1551791. eCollection 2025.
Human endometrial mesenchymal stem cells (H-EMSCs) can inhibit endometrial fibrosis and repair damaged endometrium. However, direct cell injection into dam-aged endometrium shows limited cell survival. Cell seeding onto biomaterial-based electrospun membranes could improve H-EMSCs' survival and prolong their stay at the damaged endometrium. Polycaprolactone (PCL), silk fibroin (SF) and hyaluronic acid (HA) are synthetic or natural biomaterials used by the biomedicine field, however, their effects on the biological activities of H-EMSCs remain unclear.
In this study, CD90CD73CD45 H-EMSCs were extracted from human endometrium and H-EMSCs showed enhanced adhesion, proliferation on PCL-HA vs. PCL, PCL-SF, establishing the potential of the composite to address cell survival issues.
H-EMSCs cultured on PCL-HA showed decreased IL-6 gene expression and increased IL-10, VEGFA, TGF-β gene expression vs. PCL-SF, establishing the potential to create a favorable micro-environment for generating vascularized endometrial tissues. PCL, PCL-SF, PCL-HA all supported CD90 and Meflin expression of the seeded H-EMSCs, establishing PCL as a platform to form enhanced biomaterial composites for endometrial repair in the future.
This study provided significant evidence sup-porting the potential of appropriately tailored composites of PCL and HA to moder-ate inflammation and wound-healing, which can be applied for endometrial tissue repair and regeneration.
人子宫内膜间充质干细胞（H-EMSCs）可抑制子宫内膜纤维化并修复受损子宫内膜。然而，将细胞直接注射到受损子宫内膜中显示细胞存活率有限。将细胞接种到基于生物材料的电纺膜上可提高H-EMSCs的存活率并延长其在受损子宫内膜中的停留时间。聚己内酯（PCL）、丝素蛋白（SF）和透明质酸（HA）是生物医学领域使用的合成或天然生物材料，然而，它们对H-EMSCs生物学活性的影响仍不清楚。
在本研究中，从人子宫内膜中提取CD90⁺CD73⁺CD45⁻ H-EMSCs，与PCL、PCL-SF相比，H-EMSCs在PCL-HA上表现出增强的黏附、增殖能力，确立了该复合材料解决细胞存活问题的潜力。
与PCL-SF相比，在PCL-HA上培养的H-EMSCs显示IL-6基因表达降低，IL-10、VEGFA、TGF-β基因表达增加，确立了为生成血管化子宫内膜组织创造有利微环境的潜力。PCL、PCL-SF、PCL-HA均支持接种的H-EMSCs的CD90和波形蛋白表达，确立了PCL作为未来形成用于子宫内膜修复的增强生物材料复合材料平台的地位。
本研究提供了重要证据，支持适当定制的PCL和HA复合材料在调节炎症和伤口愈合方面的潜力，可应用于子宫内膜组织修复和再生。
