Xu Dongdong, Li Zongli, Deng Zhennan, Nie Xin, Pan Yihuai, Cheng Gu
School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Int J Biol Macromol. 2024 May;266(Pt 1):131124. doi: 10.1016/j.ijbiomac.2024.131124. Epub 2024 Mar 22.
Degradation profiles are critical for the optimal application of electrospun polymer nanofibers in tissue regeneration, wound healing, and drug delivery systems. In this study, natural and synthetic polymers and their composites were subjected to in vivo transplantation and in vitro treatment with lipases, macrophages, and acetic acid to evaluate their degradation patterns. The effects of environmental stimulation, surface wettability, and polymer components on the degradation profiles of the electrospinning poly(ε-caprolactone)/silk fibroin (PCL/SF) nanofibers were first evaluated. In vivo degradation study demonstrated that bulk degradation, characterized by the transition from microfibers to nanofibers, and surface erosion, characterized by fusion between the microfibers or direct erosion from both ends of the microfibers, occurred in the electrospun membranes; however, bulk degradation dominated their overall degradation. Furthermore, the degradation rates of the electrospun PCL/SF membranes varied according to the composition, morphology, and surface wettability of the composite membranes. After the incorporation of silk fibroin (SF), the degradation rate of the SF/PCL composite membranes was faster, accompanied by larger values of weight loss and molecular weight (Mw) loss when compared with that of the pure poly(ε-caprolactone) (PCL) membrane, indicating a close relationship between degradation rate and hydrophilicity of the electrospinning membranes. The in vitro experimental results demonstrated that enzymes and oxidation partially resulted in the surface erosion of the PCL/SF microfibers. Consequently, bulk degradation and surface erosion coordinated with each other to enhance the hydrophilicity of the electrospinning membranes and accelerate the in vivo degradation.
降解特性对于电纺聚合物纳米纤维在组织再生、伤口愈合和药物递送系统中的最佳应用至关重要。在本研究中，天然和合成聚合物及其复合材料接受了体内移植以及用脂肪酶、巨噬细胞和乙酸进行的体外处理，以评估它们的降解模式。首先评估了环境刺激、表面润湿性和聚合物成分对电纺聚（ε-己内酯）/丝素蛋白（PCL/SF）纳米纤维降解特性的影响。体内降解研究表明，电纺膜中发生了以从微纤维向纳米纤维转变为特征的整体降解以及以微纤维之间融合或从微纤维两端直接侵蚀为特征的表面侵蚀；然而，整体降解主导了它们的总体降解。此外，电纺PCL/SF膜的降解速率根据复合膜的组成、形态和表面润湿性而变化。掺入丝素蛋白（SF）后，与纯聚（ε-己内酯）（PCL）膜相比，SF/PCL复合膜的降解速率更快，伴随着更大的重量损失和分子量（Mw）损失值，表明电纺膜的降解速率与亲水性之间存在密切关系。体外实验结果表明，酶和氧化部分导致了PCL/SF微纤维的表面侵蚀。因此，整体降解和表面侵蚀相互协同，以增强电纺膜的亲水性并加速体内降解。
