Yuguchi Takaaki, Dankyi Benedicta O, Rojrung Rattanaporn, Nagaoka Hikaru, Kanoi Bernard N, Tiono Alfred B, Nebie Issa, Ouedraogo Alphonse, Miura Kazutoyo, Sattabongkot Jetsumon, Sirima Sodiomon B, Tsuboi Takafumi, Takashima Eizo
Division of Malaria Research, Proteo-Science Center, Ehime University, Matsuyama, Japan.
Centre for Malaria Elimination, Institute of Tropical Medicine, Mount Kenya University, Thika, Kenya.
Front Immunol. 2025 Feb 26;16:1521082. doi: 10.3389/fimmu.2025.1521082. eCollection 2025.
Individuals residing in malaria-endemic regions with high disease transmission can develop semi-immunity within five years of age. Although understanding the target of the IgGs in this age group helps discover novel blood-stage vaccine candidates and serological markers, it has not been well elucidated due to limited accessibility to plasmodial antigens and samples. This study presents the first comprehensive analysis of antibody levels in plasma obtained from Burkinabe children (n=80, aged 0 to 5 years) to 1307 proteins expressed by the eukaryotic wheat germ cell-free system. Antibody levels were measured by AlphaScreen. We found that 98% of antigens were immunoreactive. The number of reactive antigens by the individual was correlated with increasing age. The most significant increases in seroprevalence occur during the first 2 years of life. By correlating antibody levels and the number of clinical malaria during a 1-year follow-up period, we identified 173 potential protein targets which might be associated with clinical immunity. These results provide valuable insights into how children acquired semi-immunity to malaria in their early lives.
居住在疟疾高传播流行地区的个体在五岁前可形成半免疫状态。虽然了解该年龄组中IgG的靶标有助于发现新的血液期疫苗候选物和血清学标志物，但由于疟原虫抗原和样本获取有限，这一点尚未得到充分阐明。本研究首次全面分析了从布基纳法索儿童（n = 80，年龄0至5岁）获得的血浆中针对真核小麦胚无细胞系统表达的1307种蛋白质的抗体水平。通过AlphaScreen测量抗体水平。我们发现98%的抗原具有免疫反应性。个体的反应性抗原数量与年龄增长相关。血清阳性率的最显著增加发生在生命的头两年。通过在1年随访期内将抗体水平与临床疟疾数量相关联，我们确定了173个可能与临床免疫相关的潜在蛋白质靶标。这些结果为儿童在生命早期如何获得疟疾半免疫提供了有价值的见解。
