Kolouchová Irena, Schreiberová Olga, Sigler Karel, Masák Jan, Řezanka Tomáš
Department of Biotechnology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic.
Department of Biotechnology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic
FEMS Yeast Res. 2015 Nov;15(7). doi: 10.1093/femsyr/fov076. Epub 2015 Aug 30.
The possibility of utilizing volatile fatty acids (VFA)-containing waste substrates from biotechnological and industrial processes was investigated by cultivating both oleaginous (Candida sp., Rhodotorula glutinis, Trichosporon cutaneum, Yarrowia lipolytica) and non-oleaginous (Kluyveromyces polysporus, Saccharomyces cerevisiae, Torulaspora delbrueckii) yeast species on acetic acid, propionic acid and a combination of either acid with glucose as carbon and energy sources. Both oleaginous and non-oleaginous yeasts grew on VFA. Oleaginous yeasts accumulated lipids to 15-48% of dry cell weight, non-oleaginous yeasts also grew on VFA and showed comparable biomass yields but the lipid content was only 2-5%. Biomass and lipid yield increased in cultivations on VFA plus glucose. The lipid composition was comparable to plant-derived oils and therefore might be exploitable in biodiesel production; nearly all species, when cultured on propionate, showed a high content of the desirable odd-chain unsaturated FA, especially 17:1 acid. This study points at the wide array of possible applications of many yeasts, even non-oleaginous strains, for biovalorization of industrial wastes. Despite their low lipid content these species are useful because they can readily utilize VFA from waste products and, since they are not biologically hazardous, their biomass can be afterwards used, e.g. as livestock fodder.
通过以醋酸、丙酸以及这两种酸与葡萄糖的组合作为碳源和能源，培养产油酵母（假丝酵母属、粘红酵母、皮状丝孢酵母、解脂耶氏酵母）和非产油酵母（多孢克鲁维酵母、酿酒酵母、德巴利酵母），研究了利用生物技术和工业过程中含挥发性脂肪酸（VFA）的废弃底物的可能性。产油酵母和非产油酵母都能在VFA上生长。产油酵母积累的脂质占干细胞重量的15%至48%，非产油酵母也能在VFA上生长，且生物量产量相当，但脂质含量仅为2%至5%。在VFA加葡萄糖的培养中，生物量和脂质产量增加。脂质组成与植物源油相当，因此可能可用于生物柴油生产；几乎所有菌种在丙酸盐上培养时，都显示出高含量的理想奇数链不饱和脂肪酸，尤其是17:1酸。这项研究指出了许多酵母，甚至是非产油菌株，在工业废物生物转化方面的广泛可能应用。尽管它们的脂质含量低，但这些菌种很有用，因为它们能轻易利用来自废品的VFA，而且由于它们没有生物危害性，其生物质随后可用于，例如作为牲畜饲料。
